Study on Mechanism of Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)Drug Pair in Regulating PI3K/Akt/mTOR Pathway-Mediated Cellular Autophagy to Protect Podocytes
Objective To investigate the effect of Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)drug pair on regulating autophagy of podocyte through PI3 K/Akt/mTOR signaling pathway in the treatment of diabetic nephropathy.Meth-ods Sixty successfully modeled C57BL/KSJ-db/db(hereinafter referred to as db/db)mice were randomly divided into model group,metformin group,valsartan group and Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)drug pair(low,medium and high dose)groups,10 mice in each group.Another 10 C57BL/KSJ-db/m(hereinafter referred to as db/m)mice were taken as the normal group.The mice in the normal and model groups were given physiological saline,and the mice in the treatment groups were given the corresponding drugs,and the renal pathological changes,the number and structure of autophago-somes of podocyte and the related protein expression were examined after 8 weeks of administration.Results Compared with the model group,the Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)drug pair group significantly reduced glo-merular basement membrane thickening,increased the number of autophagosomes in the podocyte,significantly increased the ex-pression of autophagy-related proteins(P＜0.05)and decreased the expression of PI3K/Akt/mTOR signaling pathway-related proteins(P＜0.05).Among them,the improvement of all indexes in the Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Lin-derae Radix)high dose group was better than that in the Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)low and medium dose groups.Conclusion The effect of Yizhiren(Alpiniae Oxyphyllae Fructus)-Wuyao(Linderae Radix)drug pair in the treatment of diabetic nephropathy is to inhibit the activation of PI3 K/Akt/mTOR signaling pathway,increase the level of autophagy in podocyte and reduce podocyte damage in mice with diabetic nephropathy.
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