Effect of Shenqi Compound(参芪复方)on mRNA and lncRNA Expression Profiles in Liver of Diabetic GK Rats
Objective To explore the mechanism of Shenqi Compound(参芪复方)regulating the expression profiles of mRNA and lncRNA in the liver of diabetic GK rats by whole transcriptome sequencing and RT-qPCR.Methods Type 2 diabetes model was prepared by high fat and high sugar diet,and the GK rats were randomly divided into model group,Shenqi Compound group and western medicine group,and fed with high-fat and high-sugar diet.In addition,another 10 Wistar rats were set as the blank group and fed with the ordinary diet.The rats in Shenqi Compound group were given Shenqi Compound extract by gavage,those in the western medicine group were given sitagliptin suspension by gavage,and those in model group and blank group were given normal saline by gavage.After 12 weeks of intervention,fasting blood glucose(FBG)was measured weekly.HE staining was used to observe the pathological morphology of liver tissue of the rats.Four rats were randomly selected from each group of the blank group,model group and Shenqi Compound group for whole transcriptome sequencing.The differential expression pro-files of mRNA and lncRNA were constructed,and the biological functions of the differential genes were analyzed and verified by RT-qPCR.Results Compared with that of the blank group,the FBG level of diabetic GK rats was significantly increased(P＜0.05).The liver of the model group showed disordered hepatocyte arrangement,blurred boundary,diffuse vacuolar changes,mod-erate steatosis,inflammatory infiltration and diffuse edema.Compared with that in the model group,the FBG level of rats in the Shenqi Compound group was significantly decreased at 12 weeks(P＜0.05),the liver tissue was arranged neatly,and steatosis,cell edema and inflammatory cell infiltration were significantly reduced.The whole transcriptome results showed that compared with those of the blank group,the mRNA and lncRNA expression profiles of the model group were significantly different,which determined the physiological and pathological differences of the diabetic rats.Shenqi Compound can widely regulate the differen-tial expressions of mRNA and lncRNA.Enrichment analysis showed that Shenqi Compound can improve diabetes by regulating multiple signaling pathways related to endocrine system and metabolic process,including insulin secretion,non-alcoholic fatty liver disease,cholesterol metabolism,sphingolipid metabolism,insulin resistance and other signaling pathways.RT-qPCR results showed that genes Irf1,Trim30,Tmcc3 and Insig1 were consistent with the transcriptome results,and the transcriptome accuracy was high.Conclusion The effect of Shenqi Compound on regulating blood sugar and improving liver lipid deposition and edema may be related to the extensive regulation of differential expressions of mRNA and lncRNA.
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