Effect of Polydatin in Huhuang Burn Limiment(虎黄烧伤搽剂)on Apoptosis,Immune Function and JNK Signaling Pathway in Rats with Skin Cancer after Radiotherapy
Objective To investigate the effects of resveratrol glycosides in Huhuang Burn Limiment(虎黄烧伤搽剂)on apop-tosis,immune function and JNK signaling pathway in skin cancer rats after radiotherapy.Methods Eighty SD rats were divided in-to a control group and a model group,20 in the control group and 60 in the model group.The the rat skin cancer model was es-tablished in the model group and the cancerous site was irradiated with 60 Gy dose radiation.The resveratrol glycosides in the Hu-huang Burn Limiment(10 mg/kg,20 mg/kg and 30 mg/kg)were administered orally to rats in the model group,which were di-vided into 10 mg/kg group,20 mg/kg group,30 mg/kg group.Flow cytometry was used to detect the apoptosis.Enzyme-linked immunosorbent assay(ELISA)was used to detect the immune function of the rats.Western blot was used to detect activated pro-teinase(JNK),phosphorylated JNK(p-JNK)and cystine aspartic acid protease-3(Caspase-3),B lymphoma-2(Bcl-2)and B lymphoma-2 related protein(Bax).Results Compared with those of the control group,the apoptosis rate in the model group was significantly reduced(P＜0.05),the expression of Bcl-2 protein was significantly increased(P＜0.05)and the ex-pression of Bax protein was significantly reduced(P＜0.05).Compared with those of the model group,the resveratrol glycosides in Huhuang Burn Limiment groups(10 mg/kg,20 mg/kg and 30 mg/kg)significantly increased the apoptosis rate(P＜0.05),significantly reduced Bcl-2 protein expression(P＜0.05)and Bax protein expression was significantly increased(P＜0.05).Compared with those of the control group,the serum levels of IL-β,IL-6 and TNF-α in the model group were significantly in-creased(P＜0.05),the activities of MDA,CK and TnⅠ were significantly increased(P＜0.05)and the activity of SOD was sig-nificantly reduced(P＜0.05).Compared with those of the model group,resveratrol glycosides Huhuang Burn Limiment groups(10 mg/kg,20 mg/kg and 30 mg/kg)significantly reduced serum levels of IL-β,IL-6 and TNF-α(P＜0.05).The activi-ties of MDA,CK and TnⅠ were significantly reduced(P＜0.05)and SOD activity was significantly increased(P＜0.05).Com-pared with those of the control group,the JNK protein level in the model group was not significantly different(P＞0.05),the p-JNK protein level was significantly increased(P＜0.05).Compared with those of the model group,resveratrol glycosides in Hu-huang Burn Limiment groups(10 mg/kg,20 mg/kg and 30 mg/kg)had no significant effect on the level of JNK protein(P＞0.05),but significantly reduced p-JNK protein levels.Conclusion Resveratrol glycosides in Huhuang Burn Limiment have in-hibitory effect on the cells of skin cancer rats after radiotherapy,increase the level of apoptosis and enhance the body's immunity,and the mechanism is related to the JNK signaling pathway.
resveratrol glycosides in Huhuang Bum Limiment(虎黄烧伤搽剂)skin cancer ratsapoptosis:immune func-tion:JNK signaling pathway
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