Quick Identification of Xiangfu(Cyperi Rhizoma)Decoction Pieces and Four Types of Vinegar-Processed Decoction Pieces
Objective To establish the fourier transform infrared spectra of Xiangfu(Cyperi Rhizoma)decoction pieces and 4 types of vinegar-processed Xiangfu(Cyperi Rhizoma)decoction pieces and quickly identify Xiangfu(Cyperi Rhizoma)decoction pieces and 4 types of vinegar-processed Xiangfu(Cyperi Rhizoma)decoction pieces.To establish high-performance liquid chromatography fingerprints of Xiangfu(Cyperi Rhizoma)decoction pieces and 4 types of vinegar-processed decoction pieces and determine the contents of-ketone,enone,5-hydroxymethylfurfural(5-HMF),p-coumaric acid,ferulic acid and luteolin in Xiangfu(Cyperi Rhizoma).Methods The infrared spectra of 25 batches of samples were established,the difference between the average infrared spectra was analyzed by OMNIC 9.2 software,and the common peak was calibrated by Spectrum for Window 3.02 software to calculate the relative peak height.Chromatographic Fingerprint Similarity Evaluation System of Traditional Chinese Medicines(2012 Edition)was used to establish the high-performance liquid fingerprint of Xiangfu(Cyperi Rhizoma)decoction pieces and four kinds of vinegar-processed decoction pieces,and the similarity evaluation was carried out to determine the num-bers of common peaks.SIMCA 14.1 software was used for the hierarchical cluster analysis,principal component analysis and or-thogonal partial least squares-discriminant analysis,and it was to determine whether the infrared spectrogram results and the high-performance liquid phase fingerprint results were mutually verified.The contents of α-gerinone,geranione,5-HMF,p-coumaric acid,ferulic acid and luteolin were determined.Results The correlation coefficient of the infrared spectra of 25 batches of samples was 0.934 7～0.982 9,and a total of 16 common peaks were calibrated,and the difference at 1500～1300 cm-1 band was obvious.The normal distribution analysis results showed that vinegar-boiled products,vinegar-fired products,vinegar-steamed products and Xiangfu(Cyperi Rhizoma)decoction pieces could be distinguished at the 2928 cm-1,1649 cm-1 and 995 cm 1 bands.The results of high performance liquid phase fingerprinting showed there were 21 common peaks in 25 batches of samples,and the similarity was greater than 0.9.Peak 3 was identified as 5-HMF,peak 7 as p-coumaric acid,peak 8 as feru-lic acid,peak 14 as luteolin,peak 19 as geranienone and peak 21 as α-geronone.The infrared spectroscopy and high-perform-ance liquid phase fingerprint hierarchical cluster analysis results,principal component analysis results,orthogonal partial least squares-discriminant analysis results were consistent,and can be clustered into five categories,and they can verify each other.The content of 5-HMF increased after processing.The contents of p-coumaric acid,ferulic acid and luteolin were the least in the decoction pieces,and they all increased after processing.Geranienone was reduced after vinegar frying,vinegar boiling,vine-gar steaming and vinegar boiling steaming,and the content was the highest in the decoction pieces The content of α-vanillone in-creased slightly after vinegar processing,and reduced after vinegar boiling,vinegar steaming and boiling.Conclusion There are obvious differences between the infrared spectra of Xiangfu(Cyperi Rhizoma)decoction pieces and the four vinegar-processed Xiangfu(Cyperi Rhizoma)decoction pieces,which can quickly identify Xiangfu(Cyperi Rhizoma)decoction piecesand the four vinegar-processed Xiangfu(Cyperi Rhizoma)decoction pieces.The established high-performance liquid phase fingerprint and content determination method are simple and accurate to operate.
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