Quality Evaluation of Xiangfu(Cyperi Rhizoma)Based on HPLC Fingerprint Combined with Multicomponent Content Determination
Objective To establish a high performance liquid chromatography(HPLC)fingerprint of Xiangfu(Cyperi Rhizoma)and comprehensively evaluate the quality of Xiangfu(Cyperi Rhizoma)from different regions and drug specifications based on the contents of four chemical components.Methods HPLC fingerprints were collected.Cluster analysis,principal component analysis and other stoichiometric methods were used to distinguish and compare the different specifications of Xiangfu(Cyperi Rhizoma).HPLC method was used to determine the contents of α-Cyperone,Cyperotundone,Lonicerin and Ferulic acid.Results The re-sults of C A and PC A analysis showed that Xiangfu(Cyperi Rhizoma)in different origins can be separately clustered into one cate-gory,and Xiangfu(Cyperi Rhizoma)in Jinhua of Zhejiang province and that in the other places had the largest difference.Xiang-fu(Cyperi Rhizoma)in Weishi of Henan province had the highest comprehensive score and the quality was better.Orthogonal partial least square-discriminant(OPLS-DA)results showed that the fingerprint of peak 13(α-Cyperone),peak 10(cypero-tundone)and peak 14 were the main differences in the quality of Xiangfu(Cyperi Rhizoma)in different origins.The contents of cyperotundone and α-Cyperone of Xiangfu(Cyperi Rhizoma)in Jinhua of Zhejiang province and Zhanjiang of Guangdong prov-ince were relatively close.The content of cyperotundone was much greater than that of Xiangfu(Cyperi Rhizoma)in Henan,and the content of α-Cyperone was much smaller than that of Xiangfu(Cyperi Rhizoma)in Henan.The contents of ferulic acid and lonicerin in different origins were not much different.After peeling,the contents of cyperotundone,α-Cyperone and lonicerin de-creased,and the content of ferulic acid increased.Conclusion There are differences in the quality of Xiangfu(Cyperi Rhizoma)in different origins,and the quality of Xiangfu(Cyperi Rhizoma)in Henan is the best.The chemical composition of Xiangfu(Cyperi Rhizoma)in before and after peeling is similar,and there is no obvious difference in species.
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