Characteristics of Intestinal Flora in URSA Patients with Kidney Deficiency and Blood Stasis and Its Correlation with Clinical Symptoms
Objective To observe the characteristics of intestinal microecology of unexplained recurrent spontaneous abortion(URSA)patients with kidney deficiency and blood stasis in non pregnant state and the difference of intestinal flora between UR-SA patients with kidney deficiency and blood stasis and normal women.To explore whether there is a certain correlation between URSA kidney deficiency and blood stasis syndrome and intestinal flora.Method The patients who visited the gynecology clinic and inpatient department of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from Septem-ber 2020 to January 2021 were collected as the research objects.The experimental group(30 cases)were non pregnant patients with URSA kidney deficiency and blood stasis type and the control group(40 cases)were normal non pregnant women.The basic information of subjects,TCM syndromes and other data were collected.The fecal samples were collected.The intestinal flora was detected by high-throughput sequencing and the qualitative and quantitative analysis was conducted.Results There were differ-ences in species composition,abundance,alpha and beta diversity between URSA patients with kidney deficiency and blood stasis and normal women.The age of URSA patients was positively correlated with Fecococcus,Clostridium,Anaerobic Bacteria and Bifidobacterium.The number of abortions was positively correlated with Roseburia and Erysipelothrichaceae,and negatively corre-lated with Eubacterium.TCM syndrome score was positively correlated with Fecococcus,and negatively correlated with Macro-globulus,Burkholderia karenii and Spirillum.The difference of Proteus in URSA kidney deficiency syndrome was statistically sig-nificant.Conclusion There is a difference in the abundance ratio of dominant gut microbiota between URSA patients with kidney deficiency and blood stasis type and normal women,and the main contributing bacteria to the difference are different.
recurrent spontaneous abortionkidney deficiency and blood stasis syndromeintestinal florarelevance
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