首页|中医滋阴潜阳法对多囊卵巢综合征伴胰岛素抵抗大鼠PI3K/Akt信号通路、性激素及胰岛素相关指标的影响

中医滋阴潜阳法对多囊卵巢综合征伴胰岛素抵抗大鼠PI3K/Akt信号通路、性激素及胰岛素相关指标的影响

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目的 探讨中医滋阴潜阳法对多囊卵巢综合征(polycystic ovarian syndrome,PCOS)伴胰岛素抵抗(insulin resist-ance,IR)大鼠的磷脂酰肌醇3激酶/蛋白激酶B(phosphoinositide 3-kinase/protein kinase B,PI3K/Akt)信号通路、性激素及胰岛素相关指标的影响.方法 以来曲唑加高脂饲料诱导建立多囊卵巢及胰岛素抵抗双重大鼠模型.采用滋阴潜阳法配合达英-35及二甲双胍干预,用免疫荧光法测定卵巢PI3K、Akt通路信号,实时荧光定量PCR检测卵巢PI3K、Akt途径信号分子mRNA表达及PI3K、Akt、胰岛素受体底物-1(insulin receptor substrate 1,IRS-1)、胰岛素受体(insulin re-ceptor,INSR)蛋白表达,性激素、胰岛素生长因子-1(insulin-like growth factor 1,IGF-1),胰岛素敏感指数(insulin sen-sitivity index,ISI),观察卵巢组织形态变化.结果 中医滋阴潜阳法能显著增加卵巢PI3K、Akt mRNA相对表达量(P＜0.05),并能显著增加卵巢PI3K、Akt蛋白表达,上调IRS-1、INSR,降低血清睾酮(testos teron,T)、促黄体生成素(luteini-zing hormone,LH)含量,调节ISI,增加卵巢颗粒细胞层数及黄体组织数量.结论 中医滋阴潜阳法可提高PI3K/Akt通路信号表达水平,改善胰岛素抵抗及卵泡颗粒细胞功能,调节促黄体生成素/卵泡刺激素(LH/FSH)比值,控制LH异常增高,调节多囊卵巢综合征及胰岛素抵抗.
Effect of Traditional Chinese Medicine"Nourishing Yin and Subduing Yang"Method on PI3K/Akt Signal Pathway,Sexual Hormones and Insulin Related Indicators in Rats with Polycystic Ovarian Syndrome and Insulin Resistance
Objective To analyze the effect of traditional Chinese medicine nourishing Yin and subduing Yang method on the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)signaling pathway,sex hormone and insulin related index in rats with polycystic ovary syndrome(PC OS)and insulin resistance(IR).Method A dual rat model of PCOS and IR was established by in-ducing with trazole and high-fat diet.Using the method of nourishing Yin and subduing Yang combined with intervention with Diane-35 and metformin,ovarian PI3K and Akt pathway signals were measured by using immunofluorescence,and real-time fluorescence quantitative PCR was used to detect ovarian PI3K and Akt pathway signal molecule mRNA expression,the protein expressions of PI3K,Akt and insulin receptor substrate 1(IRS-1),the levels of insulin receptor(INSR),sex hormones,insulin like growth factor-1(IGF-1)and insulin sensitivity index(ISI)and the changes in ovarian tissue morphology were observed.Result The method of nourishing Yin and subduing Yang could significantly increase the relative expressions of ovarian PI3K and Akt mRNA(P＜0.05),and could significantly increase the expressions of ovarian PI3K and Akt protein,up-regulate the levels of IRS-1 and INSR,reduced the levels of serum testosteron(T)and luteinizing hormone(LH)and regulate the level of ISI,and increase the number of granulosa cell layer and corpus luteum.Conclusion The method of nourishing Yin and subduing Yang can increase the signal expression levels of PI3K/Akt pathway,improve IR and follicular granulosa cell function,regulate the ratio of LH/follicle-stimulating hormone(FSH)and control the abnormal increase of LH,and regulate PCOS and IR.

nourishing Yin and subduing YangYin being not yet excess-Yang arriving firstpolycystic ovary syndrome with insulin resistanceP13 K/Akt signaling pathway

陈旭、王昕

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辽宁中医药大学附属医院,辽宁沈阳 110032

滋阴潜阳法 阴未盛-阳先至 多囊卵巢综合征伴胰岛素抵抗 PI3K/Akt信号通路

国家自然科学基金项目辽宁省自然科学基金面上项目辽宁省教育厅面上项目

820044012023-MS-226LJKZ0895

2024

10.13193/j.issn.1673-7717.2024.02.006

中华中医药学刊
中华中医药学会 ,辽宁中医药大学

中华中医药学刊

CSTPCD北大核心
影响因子：1.007
ISSN：1673-7717
年,卷(期)：2024.42(2)
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