摘要
目的 分析基于NF-κB/NLRP3/Caspase-1通路探究头穴透刺对阿尔茨海默病大鼠的干预效果.方法 选取50只SPF级大鼠,10只大鼠作为空白组,剩余40只建立阿尔茨海默病模型,将30只建模成功大鼠随机分为模型组、药物对照组、实验组各10只.空白组、模型组大鼠给予生理盐水进行灌胃,药物对照组给予盐酸美金刚片灌胃治疗,实验组大鼠进行针刺,观察大鼠病理组织、逃避潜伏期时间、跨越平台次数、氧化应激状态、炎症因子水平、NF-κB、NLRP3、Caspase-1 mRNA表达量、NF-KB/NLRP3/Caspase-1信号通路相对表达量.结果 与空白组相比,模型组、药物对照组、实验组逃避潜伏期时间增加、跨越平台次数减少,SOD、CAT、IL-10水平降低,MDA、IL-6、TNF-α、NF-κB、NL-RP3、Caspase-1 mRNA表达量、NF-κB、NLRP3、Caspase-1相对表达量升高(P＜0.05);与模型组相比,药物对照组、实验组逃避潜伏期时间降低,跨越平台次数增加,SOD、CAT、IL-10升高,MDA、IL-6、TNF-α、NF-κB、NLRP3、Caspase-1 mRNA表达量、NF-KB、NLRP3、Caspase-1相对表达量降低(P＜0.05);与药物对照组相比,实验组逃避潜伏期时间降低,跨越平台次数增加,SOD、CAT、IL-10 升高,MDA、IL-6、TNF-α、NF-KB、NLRP3、Caspase-1 mRNA表达量、NF-KB、NLRP3、Caspase-1相对表达量降低(P＜0.05).结论 头穴透刺可改善阿尔茨海默病大鼠氧化应激状态,降低炎症因子表达,其作用机制可能与NF-KB/NLRP3/Caspase-1通路有关.
Abstract
Objective To analyze the intervention effect of scalp acupoint penetrating acupuncture on Alzheimer's disease rats based on the nuclear factor kappa-B(NF-κB)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)/Caspase-1 pathway.Methods Fifty SPF-grade rats were selected,10 rats were used as the blank group,and the remaining 40 rats were used to establish Alzheimer's disease model.Thirty rats successfully modeled were randomly divided into model group,drug control group and experimental group,10 rats in each group.The rats in the blank group and model group were given normal saline by gavage,while those in the drug control group were treated with Memantine Hydrochloride Tablet by gavage.The rats in the experimental group were treated with acupuncture.The pathological tissues,escape latency,number of platform crossing,oxi-dative stress state,inflammatory factor levels,NF-κB,NLRP3 and Caspase-1 mRNA expression as well as the relative expres-sion of NF-KB/NLRP3/Caspase-1 signaling pathway were observed.Results Compared with those of the blank group,the es-cape latency time and target-platform crossing times were increased in the model group,the drug control group and the experi-mental group.The levels of superoxide dismutase(SOD),catalase(CAT)and interleukin-10(IL-10)were decreased,and the expressions of malondialdehyde(MDA),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),NF-κB,NLRP3 and Caspase-1 mRNA were increased(P＜0.05).Compared with those of the model group,the escape latency time was decreased in the drug control group and the experimental group,and the number of platform crossing was increased.The levels of SOD,CAT and IL-10 were increased,and the expressions of MDA,IL-6,TNF-α,NF-κB,NLRP3 and Caspase-1 mRNA were de-creased(P＜0.05).Compared with those of the drug control group,the escape latency was decreased and the number of platform crossing was increased in the experimental group.The levels of SOD,CAT and IL-10 were increased,and the expressions of MDA,IL-6,TNF-α,NF-κB,NLRP3 and Caspase-1 mRNA were decreased(P＜0.05).Conclusion Scalp acupoint pene-trating acupuncture can improve oxidative stress and reduce the expressions of inflammatory factors in rats with Alzheimer's dis-ease,which may be related to NF-KB/NLRP3/Caspase-1 pathway.
NETL
NSTL
万方数据