Nansheteng(Celastrus orbiculatus Thunb.)Extract Inhibits Non-Small Cell Lung Cancer Invasion and Metastasis via DJ-1/PTEN/FAK Axis
Objective To investigate the specific mechanism of inhibition of invasion and metastasis of non-small cell lung cancer by Nancheteng(Celastrus orbiculatus Thunb.)extract(COE).Methods The biological activity of COE inhibition in H1299 cells was determined by CCK-8 assay and the low toxic concentration was selected accordingly for the functional study of cell invasion and metastasis.The cell motility function was measured using wound healing assay and high-content imaging to track cell trajectories.Molecular studies were performed by Western bolt assay to investigate the specific molecular mechanism of COE inhibition of lung cancer invasion and metastasis.The grouping of the experiments was set up using a dose gradient for the control group(0.05％DMSO)and COE group(20 μg/mL,40 μg/mL and 80 μg/mL).Results COE significantly inhibited the cellular activity of H1299 in a dose-dependent manner.The results of wound healing assay showed that COE significantly inhib-ited the migratory ability of H1299 cells,and the statistical analysis showed a significant difference.Real-time dynamic tracking of cell trajectories by high-content imaging showed that COE significantly inhibited the migratory ability of H1299 cells by the concentration of 80 μg/mL,as evidenced by reduced migration speed and decreased mean square displacement.Western bolt as-says showed that COE inhibited non-small cell invasive metastasis via the DJ-1/PTEN/FAK axis.The normalized analysis of protein blots were all statistically significant.Conclusion COE inhibits non-small cell lung cancer invasion and metastasis via DJ-1/PTEN/FAK axis at low cytotoxic concentrations.
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