Intervention of Shenling Baizhu Powder(参苓白术散)on Sweet Taste Receptor in Type 2 Diabetic Zucker Rats
Objective To observe the effects of Shenling Baizhu Powder(参苓白 术散)on sweet taste receptor-related pro-teins in type 2 diabetic Zucker rats.Methods Six SPF-grade 5 weeks lean control Lean Zucker(LZ)male rats and 24 spontane-ous type 2 diabetic obese Zucker male rats were randomly divided into lean control group after 3 days of adaptive feeding,type 2 diabetes model group,low-dose Shenling Baizhu Powder treatment group,medium-dose Shenling Baizhu Powder treatment group and high-dose Shenling Baizhu Powder treatment group.The weight,food intake and random blood glucose of the rats were measured and recorded every week,and the oral glucose tolerance test(OGTT)was performed on the fourth week of inter-vention.After 4 weeks of experiment,the rats were sacrificed and samples of tongue tissue,jejunum tissue and ileum tissue were collected to detect the protein expression of glucose transporter-2(GLUT2),sweet taste receptors T1R2,T1R3 and α-gustducin were detected by Western blotting.Results In this experiment,the weight gain,random blood glucose value and food intake level of the type 2 diabetes model group were significantly higher than those of the lean control group,and the glucose tolerance was significantly lower than the lean control group.After treatment,all three dose groups of Shenling Baizhu Powder could reduce blood glucose,weight gain and food intake of type 2 diabetic Zucker rats to varying degrees,and improve glucose tolerance of type 2 diabetic Zucker rats.After 4 weeks of intervention,all three dose groups of Shenling Baizhu Powder could restore the expression levels of GLUT2,T1R2 and T1R3 and α-gustducin in the tongue,ileum and jejunum of Zucker rats with type 2 diabetes.Con-clusion Shenling Baizhu Powder has a good effect on lowering blood glucose.Shenling Baizhu Powder may improve the tongue and intestinal glucose transportation and sweetness signal transduction in type 2 diabetic rats,thereby restoring the sweetness per-ception and sweetness of the tongue and intestine.Glucose metabolism function plays an indirect role in controlling blood glu-cose.
type 2 diabetes mellitusZucker ratShenling Baizhu Powder(参苓白术散)sweet taste receptor(甜味觉受体)
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