Study on Serum Metabolomics of Diabetes-Associated Cognitive Decline Rats with Spleen-Yin Deficiency Based on High Performance Liquid Chromatography-Mass Spectrometry
Objective To observe the changes of metabolites in rats with spleen-Yin deficiency(SD)diabetes-associated cognitive decline(DACD)from metabonomics and explore the potential markers of SD-DACD and their significance.Methods The rats were used to establish the SD,DACD and SD-DACD models.High-performance liquid chromatography qua-drupole time-of-flight mass spectrometry(HPLC-Q-TOF/MS)was used to analyze the metabolites of rat serum samples.Results The serum metabolism of all groups existed significant differences.The results of this study suggested that the established method of metabolite profiling can distinguish SD,DACD and SD-DACD groups from the blank control(BC)group,as well as SD from SD-DACD,and DACD from SD-DACD.Among them,compared with that of the BC group,the pyridoxamine was up-regulated by about 24 times and 25 times in SD group and SD-DAD group,respectively,and down-regulated by about 21 times in DACD group,which could distinguish SD group from DACD group,SD-DAD group from DACD group.Pyridoxamine can be used as a potential biomarker to diagnose SD-DACD.The disorder of phenylalanine metabolic pathway involved mainly occurs between the different metabolites of SD group and SD-DACD group.Conclusion The pyridoxamine and phenylalanine metabolic pathways are involved in the pathogenesis of SD-DACD,and may be the basis of the correlation between SD and DACD.The re-sults can provide reference data for SD-DACD screening and etiological research based on metabolomics.
spleen-Yin deficiencydiabetes-associated cognitive declinemetabolomicsyndrome differentiation of tradi-tional Chinese medicinediagnosisserum
万方数据
维普
