Osthole Regulates Extracellular Matrix Expression,Inflammatory Response and Apoptosis of Degenerative Nucleus Pulposus Cells Through NF-KB Signaling Pathway
Objective To investigate the effect of osthole on extracellular matrix expression,inflammatory response and apopto-sis of degenerative nucleus pulposus cells and its potential mechanism.Methods The degraded nucleus pulposus cell model was established by oxygen glucose deprivation(OGD)culture,and the cells were divided into control group(normal culture),OGD group(OGD culture),osthole group(OGD culture+osthole treatment),PDTC group(OGD culture+NF-KB pathway inhibitor PDTC treatment)and osthole+PDTC group(OGD culture+osthole+PDTC treatment).The expressions of collagen type II(Col2al),Aggrecan,P65 and P-P65 proteins in the cells were detected by Western blot,the levels of inflammatory cytokines in-terleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the cell culture medium were de-tected by ELISA,and the apoptosis rate was detected by flow cytometry.Results Compared with those of the control group,the rel-ative expressions of Col2al and Aggrecan proteins in the OGD group were significantly decreased,the levels of IL-1,IL-6 and TNF-α in the cell culture medium were increased,the apoptosis rate was significantly increased,and the expression of P-P65 protein was significantly increased.Compared with those of the OGD group,the relative expression levels of Col2al and Aggrecan proteins in the cells of the osthole group and the PDTC group were significantly increased,the levels of IL-1 β,IL-6 and TNF-α in the cell culture medium were significantly decreased,the apoptosis rate was significantly decreased,and the expression of P-P65 protein in the cells was significantly decreased,while the changes of the above indicators in the osthole+PDTC group were the most obvious.Conclusions Osthole may inhibit the extracellular matrix degradation,inflammatory response and apoptosis of degenerative nucleus pulposus cells by inhibiting the activity of NF-kB signaling pathway.
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