Effect of Serum Containing Wendan Decoction(温胆汤)on Expressions of PI3K,Akt and mTOR in Astrocytes Under Environment of 10 mmol/L Glutamic Acid
Objective To investigate the effect of serum containing Wendan Decoction(温胆汤)on apoptosis,cell cycle and expressions of phosphoinositide 3-kinase(PI3K),protein kinase B(Akt)and mammalian target of rapamycin(mTOR)of rat as-trocytes induced by glutamate.Methods Sixty male SPF SD rats were randomly divided into 5 groups,including 20 in the normal group,10 in the clozapine group and 10 in the high,middle and low dose groups of Wendan Decoction.The normal group was giv-en 20 g/kg normal saline by gavage,the clozapine group was given 20 mg/kg clozapine technical drug by gavage,and the high,middle and low dose groups of Wendan Decoction were given 40,20 and 10 g/kg Wendan Decoction by gavage,once a day,for a total of 8 days.After the rats were killed,the blood was taken out,the serum was centrifuged,inactivated and sterilized,and EP tubes were used for equipment.The astrocytes of rats were divided into normal serum group,model serum group,clozapine drug containing serum group and Wendan Decoction high,medium and low dose drug containing serum groups.In addition to the nor-mal serum group,the other groups were given 10 mol/mL glutamic acid.After 48 hours of treatment,cell apoptosis and cell cycle were detected by flow cytometry.Western blotting and Real time PCR were used to detect the expressions of PI3 K,Akt,mTOR protein and mRNA.Results The serum containing Wendan Decoction could significantly reduce the apoptosis rate of rat astrocytes induced by glutamate(P＜0.01)and the percentage of cells in G0/G1 phase(P＜0.05),and increase the percentage of cells in S phase and G2/M phase.It decreased the protein expression ratios of P-P13K/P13K,P-ATK/AKT and P-mTOR/mTOR in cells(except the low dose group of Wendan Decoction,P＜0.05)and the mRNA expressions of PI3K,Akt and mTOR in cells(except the low dose group of Wendan Decoction,P＜0.01).Conclusion The serum containing Wendan Decoction can effective-ly regulate the expressions of PI3K,Akt and mTOR in rat astrocytes,which may be one of the mechanisms of Wendan Decoction treating schizophrenia.
万方数据
维普
