Study on Evaluation of Immunoregulatory Activity of Polysaccharides from Fangfeng(Saposhnikovia divaricata)in vitro and in vivo
Objective The immunoregulatory activity of polysaccharides from Fangfeng(Saposhnikovia divaricata)was evalua-ted in vitro and in vivo.Methods In vitro,RAW 264.7 mouse macrophage system was used to detect the proliferation of macro-phages by CCK-8 method,the phagocytosis of macrophages by neutral red staining,the NO release of macrophages by Griess method,and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)of macro-phages were measured by ELISA.In vivo experiments were carried out in immunocompromised BALB/c mice induced by cyclo-phosphamide.The phagocytosis function of polysaccharides from Fangfeng(Saposhnikovia divaricata)on reticuloendothelial sys-tem of mice was studied by carbon particle clearance test.The effect of polysaccharides from Fangfeng(Saposhnikovia divaricata)on immune organs of mice was evaluated by mouse immune organ index.The contents of immunoglobulin(IgM,IgG,IgA)and complement(C3,C4)in mice were determined by ELISA.Results The results in vitro showed that polysaccharides from Fangfeng(Saposhnikovia divaricata)could significantly improve the proliferation and phagocytosis of macrophages,and promote the release of NO,TNF-α and IL-6,but the effect on the release of IL-1β was not significant.The results of in vivo experiments showed that polysaccharide from Fangfeng(Saposhnikovia divaricata)could significantly improve the phagocytic capacity and immune or-gan index of immunosuppressed mice,and increase the contents of immunoglobulin(IgM,IgG,IgA)and complement(C3,C4)in immunosuppressed mice.Conclusion Polysaccharides from Fangfeng(Saposhnikovia divaricata)have good immunoregulatory ac-tivity both in vivo and in vitro,and participate in regulating specific and non-specific immunity of the body.
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