Study on Extraction and Separation Process of Polysaccharide from Baizhu(Atractylodes macrocephala)
Objective To optimize the extraction and separation process of polysaccharides from Baizhu(Atractylodes macro-cephala).Method The contents of polysaccharides from Baizhu(Atractylodes macrocephala)without protein removal and protein removal by Sevage method were compared.Three macroporous adsorption resins of AB-8,D101 and LX-38 were investigated based on adsorption rate and desorption rate.The purification process of AB-8 macroporous adsorption resin was studied by ad-sorption time,desorption time,sample flow rate,sample concentration,eluent dosage,eluent concentration and elution flow rate.Result The contents of polysaccharides without removing protein were(5.582±1.448 0)％,(5.407±0.766 8)％and(5.155±0.721 4)％.The contents of polysaccharides after removing protein by Sevage method were(4.658±0.155 8)％,(4.247±0.074 2)％and(4.610±0.173 4)％.The adsorption rate and desorption rate of AB-8 macroporous resin were(18.40±0.030 0)％and(91.73±0.078 1)％respectively.The purification process was as follows:adsorption time 3 h,de-sorption time 3 h,sample flow rate 1 BV/h,sample concentration 0.75 mg/mL,eluent dosage 4 BV,eluent concentration 15％ethanol,elution flow rate 3 BV/h.Conclusion The experiment provided the experimental basis for the extraction and separation of and a reference for the industrial production of polysaccharides from Baizhu(Atractylodes macrocephala).
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维普
