Study on Effect of Kuoyeshidagonglao[Mahonia bealei(Fort.)Carr.]on FABP5 Mediated Immune Cells in Liver Cancer
Objective To investigate the mechanism of action of Kuoyeshidagonglao[Mahonia bealei(Fort.)Carr.](KYSDGL)interfering with hepatocellular carcinoma through mediating the immune cells.Methods It explored the active components as well as targets of KYSDGL through network pharmacology,molecular docking and bioinformatics,and the data mining was carried on the liver cancer datasets GSE45267 and GSE89377 to analyze the mechanism of action of KYSDGL through mediating the immune microenvironment to intervene in liver cancer and verify it experimentally.Results Seven targets of KYSDGL-mediated immune cells intervention in cells were obtained.The molecular docking showed that six of them(PTGS2,FABP5,ADRB2,AGTR1,ESR1,RORA)could bind to components in KYSDGL.And all the gene expressions in the liver cancer group were significantly different compared with those in the control group,and there were strong correlations with clinical indicators of gender,cancer stage and lymph node metastasis status.The 10-year survival prognostic analysis of 6 key targets showed that only FABP5 and ESR1 had prognostic value.Further immune infiltration analysis showed that the expression level of FABP5 was significantly cor-related with immune cell infiltration.Finally,the pathological sections of THPA database verified that FABP5 protein was highly expressed in human cancer tissues and lowly expressed in control subjects.It also verified that FABP5 protein expression was down-regulated in KYSDGL-containing serum group compared with HepG2 hepatocellular carcinoma group by Western blot as-say(P＜0.05).Conclusion KYSDGL can regulate the immunity of tumors by suppressing the high expression of FABP5 and thus intervene in liver cancer.
万方数据
维普
