The main methods used for extracting Baiji(Bletilla striata)polysaccharide(BSP)include solvent extraction and auxiliary extraction.The main methods of separation and purification include activated carbon or hydrogen peroxide decoloriza-tion,Sevage deproteinization and column chromatography separation.Phenol sulfuric acid method and anthrone sulfuric acid method are commonly used in content determination.The molecular weight was determined by high performance liquid chroma-tography,infrared spectroscopy,gel permeation chromatography and other methods.Structural characterization is mainly deter-mined using liquid chromatography,infrared spectroscopy or nuclear magnetic resonance methods.BSP is a polysaccharide that has various biological activities such as antioxidant,anti-inflammatory,anti-tumor,immune regulation and promoting wound healing effects.It has broad application prospects in the field of biomedicine.BSP has good pharmacological activity,and cur-rently most of its research is on crude polysaccharides,and its pharmacological activity is still in the cellular stage.Therefore,the research on its extraction,separation,purification,structural identification and biological activity is still a hot topic of social con-cern.This article mainly reviewed the research progress in the extraction,separation,purification,structural composition and bio-logical activity of BSP and systematically analyzed it,providing a theoretical basis for the deep development and utilization of BSP and subsequent research.
Baiji(Bletilla striata)polysaccharideextractionisolation and purificationstructural characteristicsbiological activity
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