Study on Protective Effect and Mechanism of Linggui Zhugan Decoction(桂术甘汤)Pretreatment on Myocardial Ischemia Reperfusion Injury in Rats Based on Oxidative Stress and Its Related Apoptotic Proteins
Objecfive To explore the protective effect and mechanism of Linggui Zhugan Decoction(苓桂术甘汤,LGZG)on myocardial ischemia reperfusion injury(MIRI)in rats based on oxidative stress and its related apoptotic proteins.Methods Forty-five SPF male SD rats were randomly divided into sham operation group(Sham group),myocardial ischemia reperfusion group(IR group)and LGZG preconditioning group[IR+LGZG(0.68g/kg)group]with 15 rats in each group.The correspond-ing drug pretreatment was given by intragastric administration,once a day for 7 days.On the 8th day,the rat model of MIRI was established by ligating the left anterior descending branch of coronary artery(LAD).After 30 minutes of myocardial ischemia,the rats in the Sham group were subjected to IR for 24 hours.After successful modeling,the abdominal aorta serum and heart tissue of rats in each group were taken out to detect the related indexes.The changes of cardiac function were observed by electrocardio-gram,the area of myocardial infarction was observed by TTC staining,and the pathological changes of myocardial tissue were ob-served by HE staining.Biochemical and ELISA methods were used to detect the contents of myocardial enzyme damage markers troponin T(Tn-T),creatine kinase isoenzyme(CK-MB)and lactate dehydrogenase(LDH).The contents of superoxide dis-mutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in serum were detected by ELISA.The contents of apoptotic proteins Bax,caspase-3 and Bcl-2 in myocardial tissue were detected by Western blotting(WB).Results Com-pared with those in the Sham group,the ST segment of electrocardiogram(ECG)in the IR group arched upward obviously,the pathomorphology of myocardial tissue changed,the area of myocardial infarction increased significantly,the concentrations of Tn-T,CK-MB and LDH in serum increased significantly,the expression of SOD decreased significantly and the expression of MDA increased significantly in the IR group.The expressions of Caspase-3 and Bax protein were up-regulated and the expression of Bcl-2 was down-regulated in cardiomyocytes.Compared with the IR group,IR+LGZG group could reduce the cardiac injury induced by MIRI(P＜0.05),increase the expression of SOD(P＞0.05),decrease the expression of MDA(P＜0.01)and re-duce cardiomyocyte apoptosis induced by MIRI(P＜0.05).Conclusions Linggui Zhugan Decoction has a better therapeutic effect on myocardial ischemia reperfusion injury in rats.The mechanism is partly through the inhibition of oxidative stress and subse-quent cardiomyocyte apoptosis.
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