Effects of Zidianshen Decoction(紫癜肾煎剂)on PI3K/AKT and HIF-1α/VEGFA Signaling Pathway in HSPN Rats
Objective To observe the therapeutic effect of Zidianshen Decoction(紫癜肾煎剂)on Henoch-Schonlein purpura nephritis(HSPN)model rats,and to explore the mechanism based on phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)and hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor A(VEGFA)signaling pathway.Meth-ods 7 SD rats were randomly selected and divided into a normal control group.The remaining rats were used to establish the HSPN rat model using"bovine serum albumin(BSA)+lipopolysaccharide(LPS)+CCl4"combined with dried ginger.After 12 weeks,one rat from the normal control group and one rat from the model group were randomly selected,and the renal tissue was fixed and embedded.Immunofluorescence detection showed that immunoglobulin A(IgA)deposition in the renal tissue of the model group rats was accompanied by proteinuria.The normal group did not have the above manifestations,indicating that the model was successful.The successfully established HSPN rat model was randomly divided into model group,low and high dose(6.10,24.4 g/kg)groups of Zidianshen Decoction,and western medicine(3.93 mg/kg)group,with 6 rats in each group.After administration,the concentration of urinary protein was measured using bromocresol violet method,and the 24-hour urinary pro-tein quantification was calculated.Pathological and immunofluorescence detection of renal tissue in each group of rats and the ex-pressions of PI3K,AKT,HIF-1α and VEGFA in kidney tissue of SD rats were detected by Western blotting.Results Zidianshen Decoction can significantly reduce 24-hour urinary protein(P＜0.05).Compared with those of the model group,the expressions of PI3K/AKT and HIF-1α/VEGFA protein in kidney tissue of Zidianshen Decoction groups and western medicine group were significantly decreased,and the differences were statistically significant(P＜0.05).Conclusion Zidianshen Decoction can reduce 24-hour proteinuria in HSPN rats,improve the renal function and pathological damage and delay the progression of HSPN.The mechanism may be related to the regulation of PI3K/AKT and HIF-1α/VEGFA signaling pathway.
Henoch-Schonlein purpura nephritisPI3K/AKT signal pathHIF-1α/VEGFA signal pathway
万方数据
维普
