Experimental Study on Effect of Triple Needling of Huantiao(GB30)on Expressions of IL-1β,IL-6,TNF-α and GFAP in Hippocampus Japonicus of Rats with Sciatic Nerve Injury
Objective To explore the potential analgesic mechanism of triple needling of Huantiao(GB30)in treating neuro-pathic pain caused by sciatic nerve injury by observing the effect of triple needling of Huantiao(GB30)on the expressions of in-terleukin-1 β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and glial fibrillary acidic protein(GFAP)in hippocampus of rats with sciatic nerve injury.Methods Sixty SD rats were randomly divided into three groups:sham operation group,model group,triple needling group,single needling group and drug group,with 12 rats in each group.The rat sci-atic nerve injury model was prepared by clamp method.Acupuncture treatment was started on the second day of modeling,once a day,for 14 days.The changes of paw withdrawal latency(PWL)and sciatic nerve function index(SPI)of rats in each group were observed before and after intervention.The hippocampus was taken out to detect the expressions of IL-1β,IL-6 and TNF-αprotein by ELISA,and the expression of GFAP was detected by RT-PCR and immunofluorescence.Results Before treatment,compared with those in the sham operation group,the values of PWL and SPI in each group decreased(P＜0.01),and compared with those of the model group,the values of PWL and SPI in the triple needling group significantly improved after treatment(P＜0.01).ELISA,RT-PCR and immunofluorescence detection showed that the expressions of IL-1β,IL-6,TNF-α and GFAP in the model group,triple needling group,single needling group and the drug group were significantly higher than those in the sham operation group(P＜0.01).Compared with those of the model group,the expressions of IL-1β,IL-6,TNF-α and GFAP in the triple needling group,single needling group and drug group decreased significantly,and the expression in the triple needling group was lower than that in single needling and drug group(P＜0.01).Conclusion The analgesic mechanism of triple needling of Huantiao(GB30)in relieving neuropathic pain caused by sciatic nerve injury may be related to down-regulating the expressions of inflammatory factors in hippocampus,inhibiting the activation of astrocytes and reducing the degree of central pain sensitization.
sciatic nerve function indexpaw withdrawal latencyinterleukin-1βinterleukin-6tumor necrosis factor-αglial fibrillary acidic protein
万方数据
维普
