Protective Effect of Wuji Pill(戊己丸)on Indomethacin-Induced Gastric Ulcer in Rats
Objective This study aimed to investigate the protective effect of Wuji Pill(戊己丸)on indomethacin-induced gastric ulcer in rats and explore its possible mechanism.Methods A total of 36 SD rats were randomly divided into normal group,model group,positive control group(omeprazole,20 mg/kg),Wuji Pill low,middle and high dose groups,with 6 rats in each group.The rats in each group were given corresponding drugs or 0.5％carboxymethyl cellulose(CMC-Na)water solution once a day for 7 days.After the last administration for 1 hour,except the normal group,indomethacin(60 mg/kg)was given to all oth-er groups to make models.After 6 hours,the rats were killed and the blood and stomach tissues were collected.The gastric ulcer index and ulcer inhibition rate were calculated by modified Guth method,and the pathological changes of gastric tissue of rats in each group were observed and compared by HE and PAS staining.The contents of malondialdehyde(MDA),superoxide dis-mutase(SOD)and cyclooxygenase-1(COX-1)in stomach tissue,nitric oxide(NO),tumor necrosis factor-α(TNF-α),in-terleukin-6(IL-6)and interleukin-10(IL-10)in serum were determined by using the corresponding kit.TUNEL staining was used to compare the apoptosis of gastric tissue cells in each group.The expression levels of cyclooxygenase-2(COX-2)and nuclear factor kappa-Bp65[NF-κB(p65)]protein in gastric tissue were detected by immunohistochemistry.Re-sults Compared with those of the normal group,some structures of gastric mucosa,submucosa,muscular layer and serosa in the model group were damaged,and the gastric ulcer index and apoptosis percentage of gastric tissue cells were significantly increased(P＜0.01).The levels of MDA,TNF-α and IL-6 increased significantly(P＜0.01),while the levels of COX-1,NO,IL-10 and SOD decreased significantly(P＜0.01).The expressions of COX-2 and NF-κB(p65)protein increased significantly(P＜0.01).Compared with the model group,all groups can improve the gastric tissue injury.The middle and high dose groups of Wuji Pill and omeprazole group can significantly reducethe gastric ulcer index(P＜0.01),increase the ulcer inhibition rate,in-crease the expression levels of COX-1,NO and IL-10,and reduce the levels of MDA,COX-2,TNF-α,IL-6 and NF-κB(p65).All groups of Wuji Pill can significantly reduce the percentage of apoptosis in gastric tissue in rats,and the middle dose group of Wuji Pill has a good effect.Conclusion Wuji Pill has obvious protective effect on indomethacin-induced gastric ulcer in rats,and its mechanism may be related to improving oxidative stress,inflammation and reducing apoptosis.
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