Experimental Study on Tongfu Yiqi Decoction(通腑益气汤)Relieving Acute Renal Injury in Sepsis Rats
Objective To observe the protective effect of Tongfu Yiqi Decoction(通腑益气汤)on acute renal injury in septic rats.Methods Forty SD rats were randomly divided into sham operation group,model group,Tongfu Yiqi Decoction low dose group(TF-L)and Tongfu Yiqi Decoction high dose group(TF-H),with 10 rats in each group.The sham group made a sham opera-tion model,and the rats in the other groups established a model of sepsis cecal ligation and puncture.After operation,the rats in TF-L and TF-H groups were gavaged with Tongfu Yiqi Decoction at 0.275 g/kg and 1.1 g/kg respectively,and the rats in the sham group and model group were gavaged with distilled water of equal volume,once every 8 hours,for 3 consecutive days.On the 3rd day,the urinary albumin excretion rate and creatinine clearance rate of rats in each group were measured to evaluate the renal function,and the severity of the disease was evaluated from the behavioral perspective by using MSS score.The serum levels of tumor necrosis factor-α(TNF-α),interleukin-1α(IL-1α),interleukin-6(IL-6)and soluble cluster of differentia-tion14(sCD14)in rats were determined by ELISA after extraction.HE,PAS and Masson staining methods were used to observe the renal tissue morphology.The levels of malondialdehyde(MDA),catalase(CAT)and Glutathione(GSH)were measured by Western blot.Results(1)Renal function:The urinary albumin excretion rate of rats in the model group was higher than that in the sham group,while the creatinine clearance rate was lower.The urinary albumin excretion rate of rats in TF-L and TF-H groups was lower than that in the model group,and the creatinine clearance rate of rats in TF-H group was higher,and the differ-ence was statistically significant(both P＜0.05).(2)Serum inflammatory factors:The serum levels of TNF-α,IL-1α,IL-6 and sCD14 of rats in the model group were higher than those of the control group,and the serum levels of TNF-α,IL-1α,IL-6 and sCD14 of rats in TF-H group were lower than those of the model group,and the levels TNF-α,IL-1α and sCD14 of TF-L group were decreased with statistical significance(all P＜0.05).(3)MSS score:The scores of rats in the model group were higher than those in the sham group.The scores of rats in the TF-H group were lower than those in the model group,ex-cept for the reaction of opening eyes.The scores of rats in the TF-L group were lower than those in the model group,and the differences were statistically significant(both P＜0.05).(4)Renal tissue morphology:In the model group,the renal tubular lu-men expanded,the epithelial cells degenerated,the interstitial tissue had obvious collagen fiber deposition,glomerular edema and segmental glomerulosclerosis.The rats in TF-L and TF-H groups improved to some extent.The results of histological score,glomerulosclerosis index and renal interstitial fibrosis index were consistent with them.(5)Oxidative stress:The expression of MDA in renal tissue of rats in the model group was higher than that in the sham group,and the expressions of CAT and GSH were lower.The expression of MDA in renal tissue of rats in the TF-H group was lower than that in the model group,and the expres-sions of CAT and GSH were higher,while the expression of MDA in TF-L group was lower,and the difference was statistically significant(all P＜0.05).Conclusions Tongfu Yiqi Decoction can improve the acute renal injury and the overall condition of sep-tic rats through anti-oxidative stress,and down-regulate the expression levels of serum inflammatory factors.
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