Mechanism of Tangshen Dihuang Decoction(糖肾地黄汤)Delaying Diabetic Nephropathy Progression by Regulating lncRNA-ARAP1/miR-25-3p/SGLT2 Axis
Objective To explore the mechanism of Tangshen Dihuang Decoction(糖肾地黄汤)delaying the progression of di-abetic nephropathy by regulating lncRNA-ARAP1/miR-25-3p/SGLT2 axis.Methods Diabetic nephropathy(DN)model was induced by unilateral nephrectomy plus intrabitoneal injection of streptozotocin(STZ).Forty-five mice in the DN model group were selected,including 30 female CBA/J mice and 15 male DBA/2 mice,and 10 healthy mice were selected as the normal con-trol group.DN mice were divided into model group,Tangshen Dihuang Decoction high-dose group(TSDHDHD group),Tangsh-en Dihuang Decoction medium-dose group(TSDHDMD group)and Tangshen Dihuang Decoction low-dose group(TSDHDLD group),with 10 mice in each group.The pathological changes of mice were detected by HE staining.The levels of serum fasting blood glucose(FBG),triglyceride(TG)and cholesterol(TC)of mice were measured by automatic biochemical analyzer.The mRNA expressions of ARAP1,miR-25-3p,SGLT2 and Bax/Bcl-2 were analyzed by RT-PCR.The protein expressions of ARAP1,miR-25-3p,SGLT2 and Bax/Bcl-2 were analyzed by Western blotting.SPSS 23.0 statistical software was used.Re-sults In the control group,the cells were arranged neatly and completely,and there were a few adipocytes(P＞0.05).Compared with that in the control group,the cell structure in the model group was scattered,thinning from sparse,and the number of proxi-mal adipocytes increased,with statistical difference(P＜0.05).Compared with that in the model group,the number of adipocytes in Tangshen Dihuang Decoction each dose group decreased with statistical difference(P＜0.05).Compared with that in the con-trol group,IOD expression in the model group and Tangshen Dihuang Decoction each dose group was significantly increased by oil red O staining(P＜0.05).Compared with that in the model group,the IOD expression in Tangshen Dihuang Decoction each dose group was decreased under oil red O staining(P＜0.05).IOD expression in the TSDHDHD group was decreased by oil red O staining compared with that in the TSDHDMD group and TSDHDLD group(P＜0.05).Compared with that in the control group before treatment,the FBG levels in the model group and Tangshen Dihuang Decoction each dose group were increased(P＜0.05),but there was no statistical difference among model group and Tangshen Dihuang Decoction each dose group(P＞0.05).Compared with those before treatment,after 12 weeks of treatment,the FBG levels of the model group and Tangshen Dihuang De-coction each dose group were decreased(P＜0.05).Compared with those of the control group,the levels of TG and TC in the model group and Tangshen Dihuang Decoction each dose group were significantly increased(P＜0.05).Compared with those of the model group,the levels of TG and TC in the Tangshen Dihuang Decoction each dose group were decreased(P＜0.05).The levels of TG and TC in the TSDHDHD group were lower than those in the TSDHDMD group and TSDHDLD group(P＜0.05).Compared with those of the control group,the mrna levels of ARAP1,miR-25-3p and SglT2 were significantly increased in the model group and Tangshen Dihuang Decoction each dose group,while the mRNA levels of Bax/Bcl-2 were significantly de-creased(P＜0.05).Compared with those of the model group,the mRNA levels of ARAP1,miR-25-3p and SglT2 were de-creased,while the mRNA levels of Bax/Bcl-2 were significantly increased in Tangshen Dihuang Decoction each dose group(P＜0.05).Compared with those of the TSDHDMD group and TSDHDLD group,the mRNA levels of ARAP1,miR-25-3p and SglT2 were decreased in the TSDHDHD group,while the mRNA levels of Bax/Bcl-2 were significantly increased(P＜0.05).Compared with those of the control group,the protein expressions of ARAP1,miR-25-3p and SGLT2 in the model group and Tangshen Dihuang Decoction each dose group were significantly increased,while the protein expression of Bax/Bcl-2 was signif-icantly decreased(P＜0.05).Compared with those of the model group,the levels of ARAP1,miR-25-3p and SGLT2 in Tang-shen Dihuang Decoction each dose group were decreased,while the protein expression of Bax/Bcl-2 was significantly increased(P＜0.05).Compared with those of the TSDHDMD group and TSDHDLD group,the protein expressions of ARAP1,miR-25-3p and SGLT2 in the TSDHDHD group were decreased,while the protein expressions of Bax/Bcl-2 were significantly increased(P＜0.05).Conclusion Tangshen Dihuang Decoction may delay the progression of DN by regulating the lncRNA-ARAP1/miR-25-3p/SGLT2 axis.
万方数据
维普
