Molecular Mechanism of Chinese Traditional Medicine Monomer Toosendanin Mediating Growth of Breast Cancer by hsa-circ-0025583/hsa-miR-671-5p/SYNGR2 Axis
Objective To explore the molecular mechanism of hsa-circ-0025583/hsa-miR-671-5p/SYNGR2 axis regu-lating the growth of breast cancer mediated by toosendanin.Methods Forty patients with breast cancer admitted to the Breast and Thyroid Surgery Department of Nantong Third People's Hospital from April 2020 to April 2022 were selected as the study sub-jects.All patients underwent surgery in the hospital.The breast cancer and adjacent tissues of patients were taken out as the ex-perimental materials.The expression levels of hsa-circ-0025583 and SYNGR2 were determined by quantitative fluorescent pol-ymerase chain reaction(qPCR).Breast cancer cell line T47D cells were added with different concentrations of toosendanin to compare the inhibition rate of toosendanin at different concentrations on cell proliferation.The selected breast cancer cell lines were pretreated.Group A did not receive special treatment.0.5 μmol/L toosendanin was added to group B and group C trans-fected breast cancer cells,knocking out hsa-miR-671-5p and adding 0.5 μmol/L toosendanin to them.The hsa-circ-0025583 and SYNGR2 expression levels were compared.The above experiments were repeated three times.Results Compared with those in the normal adjacent tissues,the expression levels of hsa-circ-0025583 and SYNGR2 mRNA were decreased,and the difference was significant(P＜0.05).Both hsa-circ-0025583 and SYNGR2 mRNA were poorly expressed in breast cancer tissues,with a positive correlation(r=0.965,P＜0.05).With the increase of tranazmabelin concentration,the inhibition rate of breast cancer cell proliferation gradually increased,and it was statistically different from the 0 μmol/L concentration group(P＜0.05).The data was processed such that the half-inhibitory concentrations of breast cancer cells were 0.78 μmol/L and 0.49μmol/L at 24 h and 48 h,respectively.Compared with those of group A and C,the expression levels of hsa-circ-0025583 and SYNGR2 mRNA were significantly increased,and the difference was significant(P＜0.05).However,there was no significance between group A and C(P＞0.05).Compared with that of group A and C,the number of invaded cells at 24 h and 48 h was sta-tistically lower(P＜0.05).However,the number of invasive cells between group A and C had no significance(P＞0.05).Con-clusion Toosendanin regulates the levels of hsa-circ-0025583 and SYNGR2 by mediating the hsa-circ-0025583/hsa-miR-671-5p/SYNGR2 axis,thereby affecting the proliferation and invasion of breast cancer cells and inhibiting the growth of breast cancer cells.
traditional Chinese medicine monomer toosendaninhsa-circ-0025583hsa-miR-671-5pSYNGR2breast cancer
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