首页|牛蒡根多糖的结构表征及体外抗氧化、降血糖、降尿酸作用研究

牛蒡根多糖的结构表征及体外抗氧化、降血糖、降尿酸作用研究

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以牛蒡根多糖(Arctium lappa polysaccharide,ALP)为原料,通过高效凝胶渗透色谱法、紫外分光光度法、气相色谱-质谱联用法与核磁共振的方法,解析ALP结构。通过研究ALP对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH·)自由基和羟基(hydroxyl radical,·OH)自由基的清除能力,对α-葡萄糖苷酶和α-淀粉酶的抑制活性,以及对黄嘌呤氧化酶的抑制率,研究ALP的体外抗氧化活性、体外降血糖活性和体外降尿酸活性。结果表明:ALP相对分子量为1941 Da,具有果糖和葡萄糖的结构特点,ALP有较强的抗氧化能力、体外降血糖和降尿酸作用。研究结果可为牛蒡根作为功能性食品与医药保健品的开发提供科学依据。
Structural Characterization of Arctium lappa Polysaccharide and Its Antioxidative,Hypoglycemic and Uric Acid-Lowering Effects
The structural characterization of Arctium lappa polysaccharide(ALP)was analyzed by high performance gel per-meation chromatography,ultraviolet spectroscopy,gas chromatography-mass spectrometry and nuclear magnetic resonance.By studying the scavenging ability of ALP on 1,1-diphenyl-2-picrylhydrazyl(DPPH·)free radical and hydroxyl(·OH)free radical,the inhibitory activity of ALP on α-glucosidase and α-amylase,and the inhibitory rate of xanthine oxidase,the antioxi-dant,hypoglycemic and uric acid-lowering effects of ALP in vitro were studied.The results showed that the relative molecular weight of ALP was 1941 Da,which had the structural characteristics of fructose and glucose.ALP showed strong antioxidant abili-ty,hypoglycemic and uric acid-lowering effects in vitro.These results can provide scientific basis for the development of burdock root as functional food and health care product.

Arctium lappa polysaccharide(ALP)structural characterizationantioxidativehypoglycemicuric acid-lowering

王丹、尹海波、许亮、孟宪群、康廷国

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辽宁中医药大学药学院,辽宁大连 116600

牛蒡根多糖 结构表征 抗氧化 降糖作用 降尿酸作用

国家自然科学基金项目辽宁省科技厅博士启动项目辽宁中医药大学博士后启动基金项目

818743382021-BS-17921601A2014

2024

中华中医药学刊
中华中医药学会 ,辽宁中医药大学

中华中医药学刊

CSTPCD北大核心
影响因子:1.007
ISSN:1673-7717
年,卷(期):2024.42(7)