摘要
目的 通过检测血脂四项、血管内皮损伤因子、炎症相关因子,并结合前期的基因芯片结果,初步探讨人类免疫缺陷病毒(HIV)/艾滋病(AIDS)肺脾气虚证相关心脑血管疾病风险因素及机制,为中医药早期干预心脑血管疾病提供依据.方法 2020年9月—2020年11月选取河南省某地区确诊的20例HIV/AIDS肺脾气虚证患者作为研究对象,同地区20例HIV抗体阴性作为健康对照组.检测CD2、CD4+、CD8+计数,并计算CD4+/CD8+比值;检测血脂四项甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);检测氧化低密度脂蛋白(Ox-LDL)、载脂蛋白A-Ⅰ(ApoA Ⅰ)、载脂蛋白A-Ⅱ(ApoA Ⅱ)、载脂蛋白B(ApoB)、锌-α2-糖蛋白1(AZGP1)、可溶性血栓调节蛋白(sTM)、血管性血友病因子(vWF)、脂联素、瘦素(LEP)、超敏C反应蛋白(Hs-CRP)、单核细胞趋化因子1(MCP-1)、肿瘤坏死因子α(TNF-α).结果 HIV/AIDS肺脾气虚证患者与健康对照组比较CD4+降低,CD4+/CD8+比值倒置,TC降低,sTM、AZGP1、Ox-LDL、Hs-CRP升高.结论 HIV/AIDS肺脾气虚证患者存在炎性反应、血脂异常的表现,罹患心脑血管疾病风险增加.为中医药早期干预心脑血管疾病的发生提供新思路.
Abstract
Objective Through the detection of four items of blood lipid and vascular endothelial injury factors,and combined with the previous gene chip results,this study preliminarily discussed the risk factors and mechanism of Qi deficiency of lung and spleen syndrome of human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS)concerned with cere-brovascular diseases,providing a basis for the early intervention treatment of traditional Chinese medicine and the prevention and treatment of cardiovascular and cerebrovascular diseases.Methods From September 2020 to November 2020,20 HIV/AIDS pa-tients in a region of Henan Province diagnosed as Qi deficiency of lung and spleen syndrome were selected as the research ob-jects,and 20 HIV antibody negative patients in the same region were selected as the healthy control group.The counts of CD3+,CD4+and CD8+were detected and CD4+/CD8+ratio was calculated.Four items of blood lipid were tested.The levels of oxidized low-density lipoprotein(Ox-LDL),apolipoprotein A-Ⅰ(ApoA Ⅰ),apolipoprotein A-Ⅱ(ApoA Ⅱ),apolipoprotein B(ApoB),zinc-α2-glycoprotein 1(AZGP1),soluble Thrombomodulin(sTM),von Willebrand factor(vWF),adiponectin,leptin(LEP),high-sensitivity C-reactive protein(Hs-CRP),monocyte chemokine 1(MCP-1)and tumor necrosis factor alpha(TNF-α)were detected.Results Compared with the healthy control group,HIV/AIDS patients with Qi deficiency of lung and spleen syndrome had declined levels of CD4+,inverted ratio of CD4+/CD8+,declined level of TC and increased levels of sTM,AZGP1,Ox-LDL and Hs-CRP.Conclusion It showed that HIV/AIDS patients with Qi deficiency of lung and spleen syndrome manifested inflammatory reaction and dyslipidemia,which would increase the risk of cardiovascular disease.This study provides new ideas for the early intervention and treatment by traditional Chinese medicine as well as the prevention and treatment of cardi-ovascular diseases.
基金项目
国家自然科学基金项目(81873187)
国家自然科学基金项目(81804060)
中原领军人才项目(ZYYCYU2020119)
河南省科技攻关项目(212102311119)
河南中医药大学科研苗圃工程项目(MP2020-09)
NETL
NSTL
万方数据