Exploring Effect of Quzhuo Huayu Formula(祛浊化瘀方)on Gouty Arthritis
Objective To explore the effect and mechanism of Quzhuo Huayu Formula(祛浊化瘀方,QZHY)in the treatment of gouty arthritis.Methods Through TCMSP,GeneCards,DisGeNET,MalaCards,DAVID6.7 and other databases,the core compo-nents and core targets of QZHY were screened.The gene data of gouty arthritis model mice were obtained through the GEO data-base,and the differences among the expression genes in the samples were screened,and the common differential genes were ana-lyzed by GO analysis and KEGG signaling pathway enrichment.The infiltration of immune cells in the gouty arthritis group and the control group was analyzed,and the difference was compared.The results of the network pharmacological signaling pathway were compared and verified,and the results obtained were synthesized to determine the target of action and verified by animal ex-periments.Results Network pharmacology has found that IL-1 β,IL-6,TNF-α are core targets closely related to gouty arthri-tis.Based on the differential expression gene analysis of GEO database,multiple biological processes including leukocyte migra-tion,chemotaxis,cytokine production,neutrophil migration and TNF-α signaling pathway through the NF-κB pathway participa-ted in gouty arthritis,which were basically consistent with the results obtained by network pharmacology.Through immune cell in-filtration analysis,it is found that innate immune cells such as neutrophils and macrophages were the main immune cell types in-volved in gouty arthritis inflammatory response.The results of animal experiments showed that the method of QZHY could reduce the expressions of local IL-1 β,TNF-α and IL-6 in gouty arthritis model tissues and reduce the formation of extracellular trap-ping nets(NETs)of neutrophils.Conclusion QZHY might improve gouty arthritis inflammation through multi-component,multi-target and multi-pathway.
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