Mechanism of Xinguan Kangfu No.1 Prescription(新冠康复1号方)in Treatment of Pulmonary Fibrosis in Mice
Objective To investigate the intervention effect and mechanism of Xinguan Kangfu No.1 Prescription(新冠康复1号方)on pulmonary fibrosis in mice.Methods Seventy-two SPF male C57BI/6J mice were randomly divided into 6 groups:Control group(Con group),bleomycin model group(BLM group),Xinguan Kangfu No.1 Prescription low-dose group(XL group),Xinguan Kangfu No.1 Prescription medium-dose group(XM group),Xinguan Kangfu No.1 Prescription high-dose group(XH group),prednisone acetate group(P group),with 12 mice in each group.The pulmonary fibrosis model was induced by intratracheal injection of 5 mg/kg bleomycin(BLM).The serum and lung tissues of mice in each group were collected,and the pathological changes of lung were observed by HE staining and Masson staining.Reactive oxygen species(ROS)was detected,and serum oxidative stress indexes[malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)]were determined by kit.The apoptosis of lung tissue cells was observed by Tunel staining,the damage of alveolar epithelial cells caused by oxidative stress was observed by SP-C staining,the changes of alveolar epithelial cells and mitochondria were further observed by electron microscopy,and the activities of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax)and cysteine protease 3(Caspase-3)proteins were detected by Western blotting.Results The results of HE staining and Masson staining indicated that the BLM-induced pulmonary fibrosis model of mice was successfully constructed,and the pulmonary fi-brosis phenotype was significantly improved after the administration of the Xinguan Kangfu No.1 Prescription.The results of oxi-dative stress showed that Xinguan Kangfu No.1 Prescription could inhibit the production of ROS and MDA,and enhance the ac-tivities of GSH-Px and SOD.Tunel staining,SP-C staining and electron microscopy showed that Xinguan Kangfu No.1 Pre-scription could reduce the damage degree of BLM on mouse alveolar epithelial cells and protect the structure and function of mito-chondria.Western blotting test results showed that Xinguan Kangfu No.1 Prescription may up-regulate the expression of Bcl-2 protein by down-regulating the expressions of apoptosis-related proteins Caspase-3 and Bax.Conclusion By correcting oxi-dative/antioxidant imbalance,Xinguan Kangfu No.1 Prescription can improve the damage of alveolar epithelial cells,protect the mitochondrial structure and thus improve pulmonary fibrosis,providing a theoretical basis for the development of therapeutic drugs for pulmonary fibrosis.
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