箭叶淫羊藿叶片不同发育时期黄酮类成分形成与元素含量关系研究
Study on Relationship between Flavonoid Formation and Element Content in Different Developmental Stages of Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]Leaves
周省委 1陈璐 1董宁 1乔璐 1钟华 2杨林林1
作者信息
- 1. 河南中医药大学药学院,河南郑州 450046;河南省道地药材生态种植工程技术研究中心,河南郑州 450046
- 2. 平舆县农业农村局,河南驻马店 463400
- 折叠
摘要
目的 探究箭叶淫羊藿Epimedium sagittatum(Sieb.et Zucc.)Maxim.叶片不同发育时期黄酮类成分变化与元素积累的规律,揭示其黄酮类成分的形成机制.方法 以箭叶淫羊藿叶片为研究对象,将其按照不同发育时期分为t1～t6共6个时期,高效液相色谱(HPLC)测定4种黄酮类成分含量,紫外分光光度法测定总黄酮醇苷含量;实时荧光定量法(RT-qPCR)测定17个与黄酮类成分合成关键酶基因的相对表达量;电感耦合等离子体质谱法(ICP-MS)测定25种元素含量;皮尔逊(Pearson)相关性分析黄酮类成分与元素的关系.结果 淫羊藿苷含量呈现先上升后下降的趋势,朝霍定A、朝霍定B、朝霍定C、总黄酮醇苷的含量显著降低,但是5种黄酮类成分总量却显著升高,在t4和t5时期大量积累.大部分黄酮类成分合成关键酶基因表达量在t4和t5时期显著上调,在t2时期显著下调,不同基因的表达量随叶片发育而变化;叶片中元素Mg、Ca、Ti、Mn、Fe、Sr和Ba元素含量显著升高,元素K和Co含量显著降低;元素P、K、V、Cu的含量与箭叶淫羊藿中黄酮类成分含量呈显著性正相关.结论 箭叶淫羊藿叶片中黄酮类成分形成过程受到叶片发育程度及相关基因表达的影响,黄酮类成分在叶片发育后期大量积累.
Abstract
Objective To explore the changes of flavonoids and the accumulation of elements in the leaves of Yinyanghuo[Epi-medium sagittatum(Sieb.et Zucc.)Maxim.]at different developmental stages and to reveal the formation mechanism of fla-vonoids.Method The leaves of Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]were divided into six stages from t1 to t6 according to different developmental stages,the contents of four flavonoids were determined by HPLC.The contents of to-tal flavonol glycosides were determined by UV spectrophotometry.The relative expression of 17 genes related to flavonoid biosyn-thesis key enzyme genes were determined by qRT-PCR method and the contents of 25 elements were determined by ICP-MS.Pearson correlation analysis was used to analyze the relationship between flavonoids and elements.Results With the development of leaves,the contents of epimedin A,B,C and total flavonol glycosides decreased significantly while the content of icariin in-creased at first and then decreased.However,the total amount of five flavonoids increased significantly and accumulated in t4 and t5 periods.The relative expressions were significantly up-regulated at t4 and t5,and decreased significantly at t2,the expression of different genes varied with leaf development.The contents of Mg,Ca,Ti,Mn,Fe,Sr and Ba increased significantly,while the contents of K and Co decreased significantly.There was a significant positive correlation between the contents of P,K,V,Cu and the contents of flavonoids in Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.].Conclusion The formation of fla-vonoids in Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]leaves is affected by the degree of leaf development and the expression of related genes,and flavonoids is accumulated in the late stage of leaf development.
关键词
箭叶淫羊藿/黄酮类成分/基因相对表达量/元素/组间相关性分析Key words
Yinyanghuo[Epimedium sagittatum(Sieb.et Zucc.)Maxim.]/flavonoids/relative gene expression/elements/correlation analysis between groups引用本文复制引用
基金项目
国家自然科学基金项目(82104329)
河南省重点研发与推广专项(科技攻关)(222102310182)
出版年
2024
NETL
NSTL
万方数据