Antioxidant Activity and Therapeutic Effect on Ulcerative Colitis of Houpo(Magnolia officinalis)Volatile Oils before and after Sweating in Producing Area
Objective To study the antioxidant activity of Houpo(Magnolia officinalis)volatile oil before and after sweating in vitro and its pharmacological effect on ulcerative colitis mice.Methods DPPH(1,1-Diphenyl-2-picrylhydrazyl),ABTS[2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)]and total reducing power methods were used to evaluate the antioxi-dant activity of volatile oil from Houpo(Magnolia officinalis)before and after sweating.Forty-two male ICR mice were used to establish a classic ulcerative colitis(UC)mouse model by dextran sulfate sodium salt(DSS),and the differences of each thera-peutic index after administration of normal group,model group,high dose group of Houpo(Magnolia officinalis)volatile oil with sweating,low dose group of Houpo(Magnolia officinalis)volatile oil with sweating,high dose group of Houpo(Magnolia officina-lis)volatile oil without sweating,low dose group of Houpo(Magnolia officinalis)volatile oil without sweating and sulfasalazine group were recorded.Results Before and after sweating,the Houpo(Magnolia officinalis)volatile oil has certain reducing power and the ability to remove DPPH free radicals and ABTS+free radicals,which the Houpo(Magnolia officinalis)volatile oil had bet-ter ability to remove DPPH and ABTS+free radicals.Both the sulfasalazine group and the high dose group of Houpo(Magnolia of-ficinalis)volatile oil before and after sweating can ease the increase of DAI score in mice caused by DSS,restore the pathological state of intestinal tissue in DSS mice,improve spleen coefficient and colon mass per unit length,and regulate the levels of interleu-kin-6(L-6),interleukin-1 β(IL-1 β)and tumor necrosis factor-α(TNF-α),reduce the expressions of claudin-1 and Occludin in colon mucosa cells,which the effect of essential oil of Houpo(Magnolia offiicinalis)with sweating is better than that of essential oil of Houpo(Magnolia officinalis)without sweating.Conclusion The Houpo(Magnolia officinalis)volatile oil before and after sweating has the effect of treating UC and antioxidation,and the effect of the Houpo(Magnolia officinalis)volatile oil after sweating is better,which further explains the scientific nature of processing the sweat in the origin of Houpo(Magnolia officina-lis).
ulcerative colitisessential oil of Houpo(Magnolia officinalis)sweatingantioxidant
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