Content Determination of Active Components in Shanzhuyu(Corni Fructus)Charcoal and Its Hemostatic Effect
Objective To analyze the changes in the content of active ingredients in Cornus officinalis charcoal and its effect on hemostasis,laying the foundation for elucidating the mechanism of action of Cornus officinalis charcoal.Method The content changes of loganin,mononucleoside,and ursolic acid in Shanzhuyu(Corni Fructus)charcoal and Shanzhuyu(Corni Fructus)were determined by high-performance liquid chromatography.The content of polysaccharides and gallic acid in Shanzhuyu(Corni Fructus)charcoal was determined by phenol sulfuric acid method and casein phosphomolybdic acid method.The coagula-tion time,activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),and fibrinogen content(FIB)were measured in mice.Result The content of mononucleoside in Shanzhuyu(Corni Fructus)charcoal significantly de-creased,while the content of loganin and ursolic acid slightly increased,and the content of gallic acid significantly increased.The content of polysaccharides in Shanzhuyu(Corni Fructus)charcoal remained basically unchanged.In addition,the middle and low dose groups of Shanzhuyu(Corni Fructus)charcoalcan significantly reduce the coagulationtime and TT of mice.Conclu-sion Shanzhuyu(Corni Fructus)charcoal can promote coagulation in mice to a certain extent,exhibiting certain characteristics of charcoal roasting for hemostasis.This study effectively expanded the processing methods and efficacy range of Shanzhuyu(Corni Fructus)charcoal,developed the types of traditional Chinese charcoalmedicine,and has important application and theoretical value.
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