Objective To investigate the pharmacological activity of shikonin against Omicron strain and the inhibitory effect on the main protease of the novel coronavirus.Methods The antiviral activity of shikonin was tested using the Vero cell model trans-fected with the omicron BA.2.2 strain,and then nirmatrelvir was used as a positive control compound,and the inhibitory activity of shikonin against the novel coronavirus main protease was tested by using main protease fluorescence polarisation screening model in vitro.Results The extracorporal pharmacological activity test used Vero cell model showed that shikonin had the effect of inhibiting nucleic acid replication of the omicron strain,but shikonin did not show significant inhibitory activity against the main protease at the 20μM、50μM concentrations in a fluorescence polarisation screening model activity test.Conclusion Shikonin has pharmacological activity against the omicron strain at the cellular level,but it may be a non-specific inhibitor of the main prote-ase,and the mechanism of antiviral action is most likely to inhibit the novel coronavirus RNA polymerase.
关键词
紫草素/奥密克戎毒株/RNA聚合酶/主蛋白酶/荧光偏振筛选模型/分子对接
Key words
shikonin/Omicron Subvariant/RNA polymerase/main protease/fluorescence polarisation screening model/molec-ular dock
维普
万方数据