Regulatory Effects of Kushen(Sophorae Flavescentis Radix)and Its Processed Products on TLR4/MyD88/NF-κB Signaling Pathway in Mice with Damp-Heat Type of Ulcerative Colitis
Objective This study aimed to provide the reference for the clinical rational application of processed Kushen(So-phorae Flavescentis Radix)products by discussing and analyzing the effect and its possibility effect mechanism of Kushen(Sopho-rae Flavescentis Radix)and its processed products in the mouse model of damp-heat type of ulcerative colitis(UC).Methods The damp-heat mouse model was established by feeding high-sugar and high-fat diet,honey water,and a continuous high temperature and humidity environment.Then damp-heat type of UC mouse model was established by further drinking 3％dextran sulfate sodium(DSS)solution.The experimental animals were divided into blank group,model group,positive drug group(mesalazine 300 mg/kg),Kushen(Sophorae Flavescentis Radix)group,Kushen(Sophorae Flavescentis Radix)stir-fried with wheat bran group,Kushen(Sophorae Flavescentis Radix)immersed with rice water group(all drugs were administered at a 5 g/kg dose),wheat bran group and rice water group.The mice were given intragastric administration at the same time as UC modeling for 10 days.The general condition,body weight,fecal characteristics and occult blood of the mice were observed every day,and the general disease activity index(DAI)scores were performed.The mice were killed after the administration,and the colon length was measured.Hematoxylin-eosin(HE)staining was used to observe the pathological sections.The levels of the macro-phage migration inhibitory factor(MIF),tumor necrosis factor α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),interleukin-8(IL-8)and interleukin-10(IL-10)in serum,and the levels of superoxide dismutase(SOD)and malondialde-hyde(MDA)in colonic tissue of the mice were detected by enzyme-linked immunosorbent assay(ELISA).Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factor-κBp65(NF-κBp65)mRNA and protein expressions were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(Real-time PCR,RT-PCR)and Western blot.Results Compared with those of the model group,the DAI scores of Kushen(Sophorae Flavescentis Radix)and its processed product groups were decreased.The length of the colon shortening decreased(P＜0.01).The severity of colonic le-sion tissues and inflammatory cell infiltration were reduced.The contents of MIF,TNF-α,IL-6,IL-1 β and IL-8 in serum were decreased(P＜0.01),and the content of IL-10 was increased(P＜0.01).The content of MDA in colon tissue was de-creased(P＜0.01),and the level of SOD was increased(P＜0.01).The mRNA and protein relative expressions of TLR4,MyD88 and NF-κBp65 were decreased(P＜0.01).There was no significant difference of the wheat bran group and rice water group compared with the model group(P＞0.05).The effect of Kushen(Sophorae Flavescentis Radix)stir-fried with wheat bran,Kushen(Sophorae Flavescentis Radix)immersed with rice water groups were more obvious than those of Kushen(Sophorae Flavescentis Radix)group(P＜0.05,P＜0.01).Conclusion Kushen(Sophorae Flavescentis Radix)and its processed products can treat damp-heat type of UC,and the effect is better after fried with wheat bran or immersed with rice water.Its mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signal pathway,anti-oxidant stress and anti-inflammatory.
Kushen(Sophorae Flavescentis Radix)processingwheat branrice waterdamp-heat type of ulcerative colitisTLR4/MyD88/NF-κB signaling pathway
万方数据
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