Banxia Baizhu Tianma Decoction(半夏白术天麻汤)Intervenes in Myocardial Fibrosis in SHR Rats Combined with Dyslipidemia through AngⅡ/TGF-β1/smad Pathway
Objective To explore the effect of Banxia Baizhu Tianma Decoction(半夏白术天麻汤,BD)on the myocardial fi-brosis of the spontaneously hypertensive rats(SHR)fed with high fat by regulating angiotensin Ⅱ(Ang Ⅱ)/transforming growth factor-β1(TGF-β1)/smad pathway.Methods Ten Wistar Kyoto(WKY)rats were being as the control group(WKY-ND)and 48 SHR were were randomly divided into six groups(eight in each group)including hypertension(SHR-ND),high-fat hy-pertension(SHR-HF),low dose(BDL),medium dose(BDM),high dose(BDH)and western medicine(telmisartan)groups.After 1 week of adaptive feeding,all rats in the WKY-ND and SHR-ND groups were given the regular feed,while the other groups were given high-fat feed for 8 weeks.After successful modeling,the WKY-ND,SHR-ND and SHR-HF groups were given the equal volumes of physiological saline,while the other groups were given medication intervention for 12 weeks.And then,the myocardial tissue samples were taken out from the rats.The morphology of myocardial cells was observed by hematoxylin eosin staining(HE),and the contents of collagen fibers in myocardial tissue was observed by Masson staining.ELISA was used to detect the levels of Ang Ⅱ,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),nitric oxide(NO),oxidative modified low-density lipoprotein(ox-LDL),lectin like oxidized low density lipoprotein receptor 1(LOX-1)and reactive oxygen spe-cies(ROS)in the rat serums.The immunohistochemistry(1HC)method was used to detect the protein expressions of collagen type Ⅰ(Col Ⅰ),collagen type Ⅲ(Col Ⅲ)and α-smooth muscle actin(α-SMA).Western blot was used to detect the protein expressions of TGF-β1,phosphorylation signal transduction molecule 2(p-smad2),phosphorylation signal transduction mole-cule 3(p-smad3),matrix metallopeptidase 2(MMP2)and recombinant tissue inhibitors of metalloproteinase 1(TIMP1).Re-sults HE and Masson stainings showed that the myocardial cells were more uniform in size,more complete in shape,clear in nu-cleus,and reduced in collagen fiber area after administration,obviously in BDH group.Compared with those of the SHR-ND group,the levels of Ang Ⅱ,TNF-α,IL-6,ox-LDL,LOX-1 and ROS in the serum of the rats in SHR-HF group were signifi-cantly increased,while the levels of NO were significantly reduced.Compared with those of the SHR-HF group,the levels of Ang Ⅱ,TNF-α,IL-6,ox-LDL,LOX-1 and ROS in each treatment group were significantly reduced,while the levels of NO were significantly increased.Compared with the SHR-ND group,the SHR-HF group significantly increased the levels of Col Ⅰ,Col Ⅲ and α-SMA proteins in the myocardial tissue of rats.Compared with those of the SHR-HF group,the levels of Col Ⅰ,Col Ⅲ and α-SMA proteins in each treatment group were significantly reduced.Compared with those of the SHR-ND group,the protein levels of p-smad2,p-smad3 and MMP2 in the myocardial tissue of the rats of SHR-HF group were significantly increased,while the protein levels of TIMP1 were significantly reduced.Compared with those of the SHR-HF group,the levels of TGF-β1,p-smad2,p-smad3 and MMP2 in the myocardial tissue of rats in the BD group were significantly reduced,while TIMP1 was significantly increased.Conclusion BD can affect myocardial fibrosis in SHR rats fed with high fat by regulating AngⅡ/TGF-β1/smad signaling pathway.
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