Objective To study the mechanism of Guomin Decoction(过敏煎)in improving atopic dermatitis through in vitro and in vivo experiments.Methods The model of atopic dermatitis(AD)was established by repeated stimulation of 2,4-dinitro-chlorobenzene(DNCB).Loratadine Tablets and Guomin Decoction with low,medium and high doses(1.32,2.64,5.28 g/kg)were administered.The levels of total immunoglobulin E(IgE),interleukin-4(IL-4)and interleukin-13(IL-13)in serum were detected by ELISA.The expression levels of IL-4 and IL-13 in skin lesions were determined by immunohistochemistry(IHC)method.In vitro,anti-DNP-IgE/DNP-HSA was used to induce RBL-2H3 cell replication degranulation model.MTS method was used to detect the activity of Guomin Decoction on RBL-2H3.ELISA was used to detect the β-Hex enzyme release rate and the levels of histamine,leukotriene B4(LTB4),IL-4 and IL-13 in cell supernatant.The mRNA expressions of histamine,IL-4 and IL-13 were detected by reverse transcription-polymerase chain reaction(RT-PCR),and the protein ex-pressions of phosphorylation-extracellular regulated protein kinases(p-ERK)/ERK),phosphorylation-c-Jun N-terminal kinase(p-JNK)/JNK and p-p38/p38 were detected by Western blotting.Results Compared with those of the model group,the levels of total IgE,IL-4 and IL-13 in serum of AD mice in Guomin Decoction groups were significantly decreased(P＜0.05,P＜0.01),and the expressions of IL-4 and IL-13 in skin lesions of AD mice were decreased.β-HEX enzyme release rates(P＜0.05,P＜0.01)and the levels of histamine,LTB4,IL-4 and IL-13(P＜0.05,P＜0.01)were significantly decreased after degranulation of RBL-2H3 cells.The mRNA expressions of histamine,IL-4 and IL-13(P＜0.01)and protein expres-sions of p-ERK/ERK,p-JNK/JNK and p-p38/p38(P＜0.05,P＜0.01)were significantly decreased.Conclusion Guomin Decoction ameliorates atopic dermatitis by regulating Th2 type response and total IgE level in serum,possibly through IgE/FcεRI pathway inhibiting mast cell degranulosis.
万方数据
