Study on Dynamic Changes of Chemical Constituents in Bleaching Process of Baizhu(Atractylodis Macrocephalae Rhizoma)with Water of Washing Rice of Zhangbang Based on UPLC-Q-TOF-MS and Multivariate Statistical Analysis
Objective To study the dynamic changes of chemical constituents in the bleaching process of Baizhu(Atractylodis Macrocephalae Rhizoma)with the water of washing rice of Zhangbang,so as to provide reference for the rational formulation of bleaching process and the interpretation of processing mechanism.Method The raw products of Baizhu(Atractylodis Macrocepha-lae Rhizoma)and bleached products of 5 different bleaching stages(in the first and second stages,raw products were bleached with 9-fold volume of the water of washing rice for 12 h and 24 h,respectively;in the third,fourth and fifth stages,the raw prod-ucts were firstly bleached with 9-fold volume of the water of washing rice for 24 h,and then bleached with 9-fold volume of clean water for 12,24 and 48 h,respectively;the bleaching temperature was set at 26 ℃)were used as the research object.The chemical constituents were detected by using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The 0.1％formic acid aqueous solution(A)-acetonitrile(B)was used as the mobile phase for gradient elution(0～5 min,5％～30％B;5～14 min,30％～60％B;14～23 min,60％～70％B;23～31 min,70％～95％B;31～32 min,95％～5％B;32～35 min,5％B).The column temperature was 40 ℃.The injection volume was 2 μL and the flow rate was 0.3 mL·min-1.Electrospray ionization(ESI)was selected in positive ion with a scan range of m/z 50～1250.The data was analyzed by using PeakView 1.2 software,and the chemical constituents of raw products of Baizhu(Atractylodis Macrocephalae Rhizoma)and bleached products of 5 different bleaching stages were identified by reference materi-als,chemicalbook database and related literature.After normalization by MarkerView 1.2 software,multivariate statistical analysis was applied to screen the differential compounds,the changes of the relative contents of the differential compounds with different bleaching time were analyzed.Results A total of 55 chemical components were identified from raw products of Baizhu(Atractylo-dis Macrocephalae Rhizoma)and its bleached products of 5 different bleaching stages,including 40 common chemical components such as atractylenolide Ⅰ,Ⅱ,Ⅲ,etc.Among them,53,47,49,49,44 and 46 chemical components were identified from the raw products of Baizhu(Atractylodis Macrocephalae Rhizoma)and the bleached products of the first to the fifth stages.Compared with the raw products,vitexin and dihydrosyrindine were added in the bleaching process,while 9 components such as poly-l-histidine hydrochloride and uridine were not detected.However,4 components including 9-hydroxy-7-oxo-7H-furo[3,2-g]chromen-4-ylβ-D-glucopyranoside,4-octylbenzoic acid in the bleaching process showed a trend of disappearance-e-mergence.By multivariate statistical analysis,18 different compounds were screened out,among which the relative contents of at-ractylenolide Ⅰ,Ⅱ and Ⅲ increased first and then decreased with the extension of bleaching time,and all of them were the low-est in raw products,and the highest in the third stage of bleaching products.Conclusion Bleaching auxiliary materials(clean water and water of washing rice)and bleaching time were the main reasons of the changes of chemical components in the bleaching process of Baizhu(Atractylodis Macrocephalae Rhizoma)with the water of washing rice of Zhangbang.If atractylenolide Ⅰ,Ⅱ andⅢ were used as the index components and pharmacodynamic components,the reasonable bleaching process of Baizhu(Atractylo-dis Macrocephalae Rhizoma)was as follows:the raw products were firstly bleached with 9-fold volume of the water of washing rice for 24 h,and then bleached with 9-fold volume of clean water for 12 h.This experiment can provide a scientific basis for the interpretation of the scientific connotation of the processing of Baizhu(Atractylodis Macrocephalae Rhizoma)with the water of washing rice of features of Zhangbang.
Baizhu(Atractylodis Macrocephalae Rhizoma)water of washing ricebleaching processchemical constituentsultra high performance liquid chromatography-quadrupoletime-of-flight mass spectrometry(UPLC-Q-TOF-MS)multiva-riate statistical analysis
万方数据
维普
