Dose-effect Study of Buzhong Yiqi Decoction(补中益气汤)on Immune Tolerance of Spleen DCs in AIT
Objective To study the effect of Buzhong Yiqi Decoction(补中益气汤)on immune tolerance of spleen dendritic cells(DCs)in autoimmune thyroiditis(AIT)mice and its dose-effect relationship.Methods Among the 90 SPF female NOD.H-2h4 mice aged 6～8 weeks were randomly divided into normal control(NC)group,AIT group,Buzhong Yiqi Decoction high dose(BZH)group,Buzhong Yiqi Decoction middel dose(BZM)group,Buzhong Yiqi Decoction low dose(BZL)group and sodi-um selenite tablet(SS)group,with 15 mice in each group.BZH group was given 16.38 g·kg-1 Buzhong Yiqi Decoction daily,BZM group was given 8.19 g·kg-1 Buzhong Yiqi Decoction daily,BZL group was given 4.10 g·kg-1 Buzhong Yiqi Decoction daily,SS group was given 0.26 mg·kg-1 sodium selenite tablet daily,NC group and AIT group were given the same volume of distilled water daily.After 8 weeks of continuous gastric perfusion,the levels thyroglobulin antibody(TgAb),thyroid stimulating hormone(TSH)and interleukin-10(IL-10)were detected by ELISA.The expressions of MHC-Ⅱ,CD80,CD86 and CD40 on the surface of DCs in spleen of mice in each group as well as the ratio of Th17/Treg cells in spleen were detected by flow cytome-try.Results ①HE staining showed that the infiltration of thyroid lymphocytes in the AIT group was significant,and the infiltra-tion of thyroid lymphocytes in all treatment groups recovered,among which the effect of Buzhong Yiqi Decoction was mostly signif-icant in BZM group.②The levels of serum TgAb and TSH of mice in each group were detected by ELISA.It was found that the serum TgAb in the AIT group was significantly higher than that in NC group,and the other treatment groups could reduce the ser-um TgAb level,especially the BZH group,but there was no significant difference between BZH group and BZM group.Compared with those of the NC group,the serum TSH of other groups increased,but there was no significant difference among the groups.③The secretion of IL-10 in spleen of mice in the AIT group was lower than that in the NC group,while the secretion level of IL-10 in spleen was increased in other treatment groups,which was similar to TgAb results,and the effect was mostly obvious in the BZH group,but there was no significant difference between BZH group and BZM group.④Compared with those of the NC group,the expressions of spleen DCs surface factors CD40,CD80,CD86 and MHC-Ⅱ in the AIT group increased,and the levels of spleen T cells Th17/Treg increased,while the levels of spleen DCs surface factors and Th17/Treg in each treatment group de-creased.Conclusion Buzhong Yiqi Decoction can reduce the levels of thyroid autoantibodies in AIT mice,improve the pathologi-cal damage of thyroid tissue,reduce the expressions of CD80,CD86,CD40 and MHC-Ⅱ on DCs surface,promote the secretion of IL-10,maintain the balance of Th17/Treg axis and delay the occurrence of AIT.It is recommended that the best dose of mice is 9.18 g·kg-1.
万方数据
维普
