Effect of Xiaoyao Bushen Formula(逍遥补肾方)on Mechanism of Experimental Autoimmune Thyroiditis Model Rats Based on Oxidative Stress and Intestinal Flora
Objective To explore the therapeutic mechanism of Xiaoyao Bushen Formula(逍遥补肾方)by observing the effect of it on the oxidative stress and intestinal flora in experimental autoimmune thyroiditis(EAT)rats.Methods Sixty-five rats were randomly divided into the control group(10 rats)and the EAT model group(55 rats).The EAT model group used high iodine water feeding combined with subcutaneous injection of thyroglobulin to replicate the EAT model.The successfully modeled EAT rats were randomly divided into the model group,the selenium yeast(Se)group and Xiaoyao Bushen Formula group,with 17 rats in each group.The Se group was given 25.2 mg/(kg·d)selenium yeast tablet aqueous solution,the Xiaoyao Bushen Formula group was given 11.79 g/(kg·d)Xiaoyao Bushen Formula and the control group and the model group were given the equal vol-ume of distilled water by gavage.After 8 weeks of continuous intragastric administration,the levels of serum free triiodothyronine 3(FT3),free thyroxine(FT4),thyroid stimulating hormone(TSH),thyroglobulin antibody(TGAb),thyroid peroxidase antibody(TPOAb),superoxide dismutase(SOD),malonic dialdehyde(MDA)and glutathione peroxidase(GPx)of rats in each group were detected by enzyme-linked immunosorbent assay(ELISA),and the level of reactive oxygen species(ROS)was detected by im-munofluorescence.The feces of rats in each group were collected for 16S rDNA high-throughput sequencing,the changes of in-testinal flora diversity and species abundance were analyzed,and the relevant flora of Xiaoyao Bushen Formula to improve EAT rats was screened through correlation analysis.Results Compared with those of the control group,the levels of serum TSH,SOD,SOD/MDA and GPx of the model group rats were significantly reduced,while the levels of TPOAb,TGAb and MDA were marked-ly increased,and the immune fluorescence intensity of ROS was significantly enhanced.After administering treatment drugs,the above indicators in the Xiaoyao Bushen Formula group and Se group improved compared to those of the model group(P＜0.01 or P＜0.05).16S rDNA sequencing results showed that Xiaoyao Bushen Formula could regulate the relative abundance of Bacillo-ta,Bacteroidota and other bacteria to regulate the disorder of intestinal flora in EAT rats.Desulfovibrio and Coprococcus were the dominant bacteria in Xiaoyao Bushen Formula group.Conclusion Xiaoyao Bushen Formula can adjust the serum TSH and thyroid double antibody level of EAT rats and improve the oxidative stress state,and its mechanism may be related to regulating the im-balance of various intestinal flora.
万方数据
维普
