Effect of Maidong(Radix Ophiopogonis)on TRPA1,SP and CGRP in Mice with Cough after Mycoplasma Pneumoniae Infection
Objective It investigated the inhibitory effects of Maidong(Radix Ophiopogonis)on cough in mice after Mycoplas-ma pneumoniae infection by regulating the expression of transient receptor potential A1(TRPA1),substance P(SP)and calcito-nin gene related peptide(CGRP).Methods The affinity of Maidong(Radix Ophiopogonis)with cough factor TRPA1 was detected by Biomolecular interaction analysis(Biacore)technique and verified by in vivo pharmacodynamics.The experiment was divided into blank group,model group,pentoxifylline group,Maidong(Radix Ophiopogonis)high dose group,Maidong(Radix Ophiopogo-nis)medium dose group and Maidong(Radix Ophiopogonis)low dose group,10 mice in each group.Except the blank group,the BALB/c mice of the remaining 5 groups were modeled by nasal drip of 20 μL 106 CCU mycoplasma pneumoniae for 3 d.The mice in each group were given the corresponding drugs by gavage for 7 d.Cough elicitation experiments were performed to ob-serve the coughing of mice in each group within 3 min.HE and Masson staining were performed to observe the pathological chan-ges of lung tissues and immunohistochemistry was performed to observe the expressions of TRPA1,SP and CGRP in the lung tis-sues of mice in each group.The mRNA expressions of TRPA1,SP and CGRP in the lung tissues of mice were detected by Real-time PCR.Results Compared with the model group,the Maidong(Radix Ophiopogonis)high dose group significantly reduced the number of coughs in mice.Hematoxylin-eosin(HE)staining and Masson staining showed that a large number of inflammatory cells infiltrated the bronchi and alveoli and collapsed the alveolar wall in the model group,while the histopathological changes in the Maidong(Radix Ophiopogonis)administration groups were reduced compared with those in the model group.Immunohisto-chemistry and Real-time PCR results showed that the expressions of TRPA1,SP and CGRP mRNA in the lung tissues of mice in the Maidong(Radix Ophiopogonis)high and medium dose groups decreased.Conclusion Maidong(Radix Ophiopogonis)was a-ble to reduce the expressions of TRPA1,SP and CGRP in the lung tissue of mice with cough after mycoplasma pneumoniae infec-tion,thus achieving the treatment of cough after mycoplasma pneumoniae infection.
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