Effect of Gancao Xiexin Decoction(甘草泻心汤)on Gut Microbiota and Th17/Treg Axis in Patients with Inflammatory Bowel Disease and Chronic Periodontitis
Objective To investigate the effect of Gancao Xiexin Decoction(甘草泻心汤)on gut microbiota and T-helper 17(Th 17)/T-regulatory(Treg)cell axis in patients with inflammatory bowel disease and chronic periodontitis.Methods The clini-cal data of 63 patients with inflammatory bowel disease and chronic periodontitis in the hospital from March 2019 to June 2013 was retrospectively analyzed.The conventional group was given conventional periodontal basic treatment and symptomatic treat-ment,and the decoction group received conventional periodontal basic treatment combined with Gancao Xiexin Decoction.Then the clinical efficacy,gingival condition,TCM syndrome scores,gut microbiota,inflammatory cytokine levels,the proportion of Th 17 and Treg cell,Th17/Treg ratio and incidence of adverse reactions were compared between two groups.Results The decoction group reported a higher clinical efficacy rate than the conventional group(96.88％vs 74.19％,P＜0.05).After treatment,the plaque and gingival index reduction was observed in all patients,and the reduction was more notable in the decoction group than that in the conventional group(P＜0.05).TCM syndrome scores showed a decrease in all patients,and the score of the decoction group was lower than that of the conventional group(P＜0.05).An increase in Bifidobacterium and Lactobacillus counts and a decrease in counts of Escherichia coli and Enterococcus were measured in all patients,and both improvements were more remark-able in the decoction group than that in the conventional group(P＜0.05).After treatment,the levels of matrix metalloproteinase(MMP8),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and interleukin-17(IL-17)in gingival crevicular fluid inflammatory decreased in both groups compared to those before treatment,and the levels in the decoction group were lower than those in the conventional group(P＜0.05).A reduction in proportion of Th17 and Th17/Treg ratio along with an increase in pro-portion of Treg was found in all patients,and the decoction group had smaller proportion of Th17 and Th17/Treg ratio,and larger proportion of Treg compared to the conventional group(P＜0.05).No statistical difference was demonstrated in adverse reaction rate between two groups(12.91％vs 9.38％,P＞0.05).Conclusion Application of Gancao Xiexin Decoction in patients with in-flammatory bowel disease and chronic periodontitis can effectively ameliorate the clinical symptoms,reduce TCM syndrome scores,improve the gut microbiota and restore Th17/Treg imbalance.
Gancao Xiexin Decoction(甘草泻心汤)inflammatory bowel diseaseperiodontitisgut microbiotaregulatory T cellsT-helper 17
万方数据
维普
