Study on Quantity Value Transfer of Standard Decoction in Raw and Processed Niuxi(Achyranthis Bidentatae Radix)
Objective To establish efficient high-performance liquid chromatography(HPLC)fingerprints for the standard de-coction of raw and processed Niuxi(Achyranthis Bidentatae Radix)and study the transfer of index components.Methods The chromatographic column was Venusil C18(250 mm×4.6 mm,5 μm)column.The column temperature was 30 ℃,and the detec-tion wavelength was 230,280 nm.The injection volume was 20 μL and the flow rate was 0.8 mL·min-1.Chromatographic Fin-gerprint Similarity Evaluation System of Traditional Chinese Medicines(2012 Edition)was used to evaluate the similarity and de-termine the common peak.The contents of index components were determined,and the transfer rate and extraction rate were cal-culated.Results The similarity of all 45 batches of samples was above 0.934.Forty-five batches of samples were calibrated with 10 common peaks,referring to the recognition of 7 peaks,namely 5-hydroxyfurfural,polypodine B,β-ecdysone,25 R-inokos-teron,25S-inokosteron,ginsenoside R0 and chikusetsu saponin Ⅳa.The average transfer rates of the seven components in the standard decoction of the raw and processed products of Niuxi(Achyranthis Bidentatae Radix)were 291.53％,28.90％,56.74％,54.61％,53.01％,64.28％,61.87％and 192.21％,61.16％,56.10％,54.69％,51.80％,58.06％,43.70％and 111.29％,53.59％,62.47％,60.84％,57.77％,50.20％,48.69％.The average extraction rate of the standard decoction of the raw and processed products of Niuxi(Achyranthis Bidentatae Radix)of standard decoction was 35.06％,34.02％and 36.32％.Conclusion The fingerprint method is stable and reliable,and it can fully demonstrate the transfer of index components during sample preparation,which can provides a reference for the study of classic prescriptions and compound preparation contai-ning Niuxi(Achyranthis Bidentatae Radix).
万方数据
维普
