Protection of icariin against H2O2 induced endoplasmic reticulum stress in PC12 cells
Objective To investigate the effect of icariin (ICA) on H2O2 induced endoplasmic reticulum (ER) stress in PC12 cells.Methods H2O2 was used as an inducer of ER stress,and PC12 cells were pre-treated with various concentrations of ICA (6.25,12.5 and 25 μM).Cell proliferation and death were detected using MTIand LDH assays.The protein expressions of GRP78,PERK,eIF2α,ATF4 and CHOP,as well as the phosphorylation levels of PERK and eIF2α were tested by Western blot.The mRNA level of GRP78,PERK,ATF4 and CHOP were measured by real-time qPCR analysis.Results The data demonstrated that H2O2 induced PC12 cells loss and ER stress.However,pre-treatment with ICA enhanced PC12 cell viability and repressed cells loss;decreased the levels of PERK,eIF2α phosphorylation and the expressions of GRP78,ATF4,and CHOP protein;as well as reduced GRP78,ATF4 and CHOP mRNA levels in H2O2-injuried PC12 cells.Conclusion The present study revealed that ICA exerts protective effect on H2O2-induced ER stress in PC12 cells,mainly related to suppression of the PERK signaling pathway activation.
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万方数据
维普
