Effects of icariside Ⅱ on hepatic cytochrome P450 expression in mice
Objective To investigate the effects of icariside Ⅱ (ICS Ⅱ) on the contents of hepatic cytochrome P450 (CYP450) enzymes and its major isoforms expressions in mice.Methods Male C57BL/6 mice were randomly divided into six groups:normal saline (NS),ICS Ⅱ (5,10 and 20 mg/kg) groups,dexamethasone (DEX,80 mg/kg) and erythromycin (ERY,200 mg/kg) groups (n =12).Mice were treated with different doses of ICS Ⅱ by garage daily for 14 days,and the NS group was administered with volume-matched saline.To induce and inhibit selectively certain CYP3A11,mice were oral gavage with either DEX and ERY,respectively.The contents of CYP450 enzymes in liver were detected by Elisa kits.Liver injury were evaluated by analyzing the histopathological changes and the level of serum biochemical parameters was detected by hematoxylin and eosin staining and Elisa kits.Western blot assay was used to analyze the expression of major CYP450 isoforms.Results ICS Ⅱ decreased the content of liver CYP450 enzymes.Furthermore,ICS Ⅱ decreased CYP3A11 and induced CYP1A2 protein expression.No obvious changes in serum and liver tissue biochemical parameters were found and no significant pathological changes were observed in liver tissues after ICS Ⅱ treatment.Conclusion The present study indicates that ICS Ⅱ could decrease the content of liver CYP450 enzymes and decrease the expression of CYP3A11 and increase the expression of CYP1A2.
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