Phylogenetic characteristics and population genetic diversity of 15 autosomal STR loci in Tujia population of Guizhou of China
Objective To investigate the genetic polymorphisms of 15 STR loci in the Tujia ethnic minority of Guizhou province,and explore the genetic relationship with other populations at home and abroad.Methods Fifteen STR loci (CSF1PO,D3S1358,D5S818,D7S820,D8S1179,D13S317,D16S539,D18S51,D21S11,FGA,TH01,TPOX,vWA,D2S1338 and D19S433) were amplified in 353 unrelated healthy Tujia population residing in northern Guizhou.Modified-Powerstates and Arlequin were used to calculate the allele frequencies and forensic parameters.Phylip,Mega and SPSS software were applied to structure population genetic relation.Results No evidence of deviation from the Hardy-Weinberg equilibrium (HWE) was identified.A total of 392 alleles was founded and the allelic frequencies spanned from 0.0014 (D7S820) to 0.5227 (TPOX).The cumulative power of discrimination (CDP) and the cumulative probability of exclusion (CPE) of 15 STR loci were 0.99999999999999999541338655 and 0.999998251,respectively.Genetic distance,N-J tree and MDS revealed that the farthest genetic distance was between Guizhou Tujia and Argentina population,whereas the nearest was among Guizhou Tujia and Hubei Tujia.Conclusion These 15 STR loci have high polymorphisms,which could be utilized as a powerful tool for forensic paternity test and personal identification in Guizhou Tujia population.The population comparison demonstrates that Guizhou Tujia had a close genetic relationship with adjacent geographic Hubei Tujia and Han populations,whereas Guizhou Tujia was obviously different from Argentina population.
forensic scienceshort tandem repeatgenetic polymorphismsGuizhou Tujia population
NETL
NSTL
万方数据
