首页|Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation"π"structuring module with angiogenesis and neurogenesis effects

Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation"π"structuring module with angiogenesis and neurogenesis effects

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
OBJECTIVE:To explore the functional role of the drug-dependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ischemia.METHODS:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric module.The biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence labeling.RESULTS:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular region.Thus,we obtained a"π"structuring module considered a neural regeneration module.The biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also verified.CONCLUSION:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.

brain ischemiastrokeneurogenesisangiogenesismodular pharmacologyMet-axiation allosteric module

CHENG Kunming、YUAN Jianan、LIU Jun、ZHANG Shengpeng、XU Qixiang、XIE Yong、ZHAO Jingfeng、ZHANG Xiaoxu、TANG Xudong、ZHENG Yongqiu、WANG Zhong

展开 >

Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui,Teaching and Research Section of Traditional Chinese Medicine,School of Pharmacy,Wannan Medical College,Wuhu 241000,China

Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Department of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China

Department of Gastroenterology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

展开 >

National Major Scientific and Technological Special Project for"Significant New Drugs Development"Joint Innovation Project of China Academy of Chinese Medical SciencesUniversity Collaborative Innovation Project of AnhuiUniversity Natural Science Research Project of Anhui Province

2017ZX09301059ZZ11-026GXXT-2020-0242023AH051752

2024

10.19852/j.cnki.jtcm.2024.01.004

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(1)
  • 26