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中医杂志(英文版)
中医杂志(英文版)

Cao Hong-xin

季刊

0255-2922

info@journaljtcm.com;jtcm@188.com

010-64050201

100700

北京东直门内南小街16号

中医杂志(英文版)/Journal Journal of Traditional Chinese MedicineCSCDSCI
正式出版
收录年代

    Network Meta-analysis of the clinical efficacy and safety of kidney-tonifying and bone-strengthening therapies for the treatment of rheumatoid arthritis with kidney deficiency type

    GAN ChangTAO QingwenYI HaoyingBIAN Yuting...
    1067-1081页
    查看更多>>摘要:OBJECTIVE:To compare the efficacy and safety of different Traditional Chinese Medicine(TCM)-based kidney-tonifying and bone-strengthening therapies for rheumatoid arthritis(RA).METHODS:The current Bayesian network Meta-analysis compared different TCM-based kidney-tonifying and bone-strengthening therapies for RA.Eight electronic databases and two trial registration platforms were searched.RevMan 5.4 and Stata 16 software were employed.RESULTS:A total of 42 studies involving 3779 cases were included.The results showed that the most effective intervention for improving efficacy was Wanbi decoction(顽痹汤);Bushen Quhan Zhiwang decoction(补肾祛寒治尪汤)was superior in reducing the incidence of adverse reactions;and Qiwei Tongbi oral liquid(七味通痹口服液)effectively reduced C-reactive protein and erythrocyte sedimentation rate levels.CONCLUSION:Combining TCMs with conventional Western Medicine can improve the clinical efficacy of RA treatments,reduce inflammatory indexes,and result in a higher safety profile.However,this conclusion needs to be verified by more high-quality randomized controlled trials.

    Chinese patent medicine for atherosclerosis:a systematic review and Meta-analysis of randomized controlled trials

    XU JianLIU YuntaoLUO ZhihaoZHAO Zhen...
    1082-1090页
    查看更多>>摘要:OBJECTIVE:To synthesize the evidence from randomized controlled trials(RCTs)to assess the efficacy and safety of Chinese patent medicine(CPM)on atherosclerosis(AS)or with a high risk of atherosclerosis.METHODS:All RCTs in three databases(PubMed,EMBASE,and Cochrane Library)were included from the inception of the database to September 20,2019.The methodological evaluation of the included trials was carried out using the Cochrane Collaboration Risk of Bias Tool.Meta-analysis was conducted using RevMan 5.3 software.The Grading of Recommendations Assessment,Development and Evaluation(GRADE)methodology was used to evaluate the quality of evidence.RESULTS:Eighteen RCTs were included,involving a total of 3885 patients with AS or with a high risk of AS.Most trials had favorable methodology.Meta-analysis suggested significant differences in clinical endpoint(n=1938,RR 0.53;95%CI 0.40 to 0.69,P<0.00001;I2=0%);the change in carotid artery IMT(n=1723,MD-0.09,95%CI-0.14 to-0.04,P<0.001;I2=40%);change in FMD(n=239,MD 0.87,95%CI 0.52 to 1.21,P<0.00001;I2=0%);change in high sensitive C-reactive protein(hs-CRP)(n=1527,MD-1.89,95%CI-3.36 to-0.42,P=0.01;I2=94%)and incidence of total adverse events(RR 0.76,95%CI 0.62 to 0.93,P=0.009;I2=40%)in favor of the experimental group.However,meta-analysis showed no significant differences in the change in low-density lipoprotein-C(LDL-C)(n=2419,MD-0.19,95%CI-0.50 to 0.12,P=0.22,I2=94%)between the experimental and control groups.Conclusion:CPM could have certain clinical efficacy in the treatment of AS.However,more double-blinded placebo-controlled RCTs are required in further evaluations to provide stronger evidence.

    Acupuncture reduces sedative and anaesthetic consumption and improves pain tolerance in patients undergoing colonoscopy:a Meta-analysis

    WANG BingyuJIN FangfangGAO JiaweiYANG Liuxin...
    1091-1103页
    查看更多>>摘要:OBJECTIVE:To evaluate the effects of acupuncture anesthesia on the consumption of sedatives and anesthetics,pain,and time consumption in patients undergoing colonoscopy,thus providing evidence that acupuncture anesthesia should be extended to endoscopists and anesthetists.METHODS:Four English and four Chinese databases were searched for randomised controlled trials of acupuncture anaesthesia in patients undergoing colonoscopy,published from database inception to 1 March 2023.Outcomes were consumption of sedatives and anaesthetics,pain tolerance,visual analog scale(VAS)score,the meantime consumption of examination,satisfaction,and adverse reactions.RESULTS:Thirty-one randomized controlled trials with a total of 4790 participants were included.Results showed that acupuncture anaesthesia significantly reduced consumption of sedatives and anaesthetics[9 studies,n=944,standardized mean difference(SMD)=-0.82,95%CI(-1.31,-0.33),P=0.001],VAS score[9 studies,n=1790,mean difference(MD)=-1.13,95%CI(-1.70,-0.57),P<0.001],meantime consumption[21 studies,n=3799,MD=-2.09,95%CI(-3.15,-1.03),P<0.001]and adverse reactions of colonoscopy[7 studies,n=738,odds ratio(OR)=0.17,95%CI(0.10,0.28),P<0.001].Acupuncture also significantly improved pain tolerance[14 studies,n=1661,OR=7.05,95%CI(3.79,13.12),P<0.001],while no beneficial effects were found for satisfaction[7 studies,n=843,SMD=0.02,95%CI(-0.38,0.43),P=0.91].CONCLUSION:Acupuncture has beneficial effects on patients undergoing colonoscopy,particularly in reducing consumption of sedatives and anaesthetics,alleviating pain,shortening the time consumption of examination and preventing adverse events.As an alternative,effective,inexpensive,and accessible approach,acupuncture anaesthesia should be extended to endoscopists and anaesthesiologists.

    2-hydroxy-3-methyl anthraquinone promotes apoptosis and inhibits invasion of human hepatocellular carcinoma cells by targeting nicotinamide adenine dinucleotide-dependent protein deacetylase sirtuin-1/cellular tumor antigen p53 signaling pathway

    WU ShuangLI QiaoZHU XieyingZHANG Taoyuan...
    1104-1110页
    查看更多>>摘要:OBJECTIVE:To investigate the anti-liver cancer effect of 2-hydroxy-3-methyl anthraquinone(HMA)and the specific mechanism based on nicotinamide adenine dinucleotide-dependent protein deacetylase sirtuin-1(SIRT1)/cellular tumor antigen p53(p53)pathway.METHODS:Cell counting kit-8 method was used to observe the effect of HMA on the activity of human hepatocellular carcinoma cells(HepG2)cells.At 72 h and 80 μL HMA,the apoptosis rate of HepG2 cells in each group was measured by flow cytometry.Transwell was used to assay for cell invasion.The protein expression levels of SIRT1,p53,B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),caspase-9(CASP9)and caspase-3(CASP3)were detected by Western Blot.RESULTS:HMA significantly inhibited the proliferation of HepG2 cells,The half inhibiting concentration(IC50)of the HMA at 24,48 and 72 h were examined and it were 126.3,98.6,and 80.55 μM,respectively.Compared with the control group,the apoptosis rate of HMA,Selisistat(EX527),and HMA+EX527 groups enhanced,while the apoptosis rate of SRT1720 diminished,demonstrating that inhibition of SIRT1 can lead to apoptosis of HepG2 cells.HMA+EX527 group had the highest apoptosis rate,the lowest expression of SIRT1 and Bcl-2,and the highest expression of p53,Bax,CASP9 and CASP3.The number of invasions of HepG2 was significantly reduced after HMA and EX527 intervened.Western blot shows HMA could inhibit SIRT1,promote the expression of p53,and decrease the ratio of Bcl-2/Bax.CONCLUSIONS:HMA induced apoptosis in HepG2 cells,while inhibiting proliferation and invasion.The mechanism of HMA against HCC may be related to the SIRT1/p53 pathway.

    Multi-omics analysis reveals the neuroprotective effect of Atractylodis Rhizoma Alba extract against Parkinson's disease in mouse

    KANG SohiLEE SueunMOON Byeong CheolSONG Jun Ho...
    1111-1117页
    查看更多>>摘要:OBJECTIVE:To assess Atractylodis Rhizoma Alba extract(ARE)neuroprotective function in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice and related genes.METHODS:Examined mRNA-DNA methylation changes induced by ARE in MPTP-induced Parkinson's disease(PD)model's substantia nigra.RESULTS:ARE mitigated MPTP-induced motor impairment in rotarod and open field tests and preserved tyrosine hydroxylase-positive neuronal cells in substantia nigra and striatum.Genome RNA-Sequencing and Methyl-Sequencing in substantia nigra of vehicle/ARE-treated MPTP-induced PD mice showed 84 differentially expressed genes(DEGs)and 1804 differentially methylated regions(DMRs).Upregulated genes involved zinc ion homeostasis,cilium protein localization,and transcription;downregulated genes linked to ephrin receptor signaling,somitogenesis,and gene expression regulation.Hyper/hypomethylated DMRs post-ARE treatment associated with Wnt signaling,mitochondrial organization,dopamine biosynthesis,and hindbrain development.No significant correlation between DEGs and methylated genes related to PD pathogenesis.CONCLUSION:This research has identified the epigenetic targets of ARE's therapeutic action and gives insight on how ARE protects neurons in Parkinson's disease.

    Renshen(Radix Ginseng)polysaccharide promotes repair of the mice intestinal mucosa through regulatory mechanisms based on polyamine and human antigen R

    WANG GuanyuDAI XingzhenLIU YitingZHU Zeming...
    1118-1126页
    查看更多>>摘要:OBJECTIVE:To investigate the mechanism and effect of Renshen(Radix Ginseng)polysaccharide on the migration of intestinal epithelial cell line 6(IEC-6),as well as the repair mechanism of Renshen(Radix Ginseng)polysaccharide on colonic injury induced by dextran sulfate sodium(DSS)in mice.METHODS:Mice were fed 3%(w/v)DSS for 6 d to create colonic lesions.A cell-migration model was created using cell scratching.mRNA expression,protein expression,translation efficiency of mRNA,and nucleoplasmic distribution of human antigen R(HuR)were determined by real-time reverse transcription-quantitative polymerase chain reaction,western blotting,a dual luciferase reporter system,and immunofluorescence staining,respectively.RESULTS:Renshen(Radix Ginseng)polysaccharide promoted the migration of IEC-6 cells and affected expression of stromal interaction molecule 1(STIM1)and cell division cycle 42(Cdc42)at transcriptional and post-transcriptional levels.CONCLUSIONS:Renshen(Radix Ginseng)polysaccharide-induced repair of intestinal mucosal injury may be mediated by increased cell migration via polyamine-based regulatory mechanisms.In vitro and in vivo experiments suggest that Renshen(Radix Ginseng)polysaccharide-induced post-transcriptional regulation of STIM1 and Cdc42 may be related to differences in the regulation of different target genes by HuR.Taken together,these data provide a reference for further exploration of the protective effect of Renshen(Radix Ginseng)on the intestinal mucosa.

    Cardioprotective mechanism of Qixuan Yijianing(芪玄抑甲宁)formula in Graves'disease mice using miRNA sequencing approach

    GAO ChangjiuDING SongShadi A.D.MohammedLU Fang...
    1127-1136页
    查看更多>>摘要:OBJECTIVE:To investigate the mechanism of Qixuan Yijianing(芪玄抑甲宁,QYN)in minimizing cardiac injury in Graves'disease(GD)mice using microRNA(miRNA)sequencing analysis.METHODS:Female BALB/c mice were randomly divided into the modeling and control groups(CG).The modeling group was established with Ad-TSHR289.Following 10 weeks of successful modeling,the mice were randomly assigned to four groups:model(MG),methimazole(MMI),QYN low-dose(LD),and high-dose(HD).After four weeks of treatment,the heart rate,heart volume,and heart index were measured,and the levels of aspartate aminotransferase(AST),lactate dehydrogenase(LDH),α-hydroxybutyrate dehydrogenase(α-HBD),creatine kinase(CK),and creatine kinase MB isoenzyme(CK-MB)in the serum were detected using a biochemical analyzer.Hematoxylin-eosin and Masson staining were used to determine histological changes in cardiac tissue.The heart tissues in the CG,MG,and HD groups were selected,and miRNA sequencing was used to identify differentially expressed miRNAs.A bioinformatics database was used to predict the target genes of differential miRNAs,and Gene Ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were conducted on the predicted target genes.RESULTS:As compared to the CG group,the MG group's heart rate,heart volume,heart index,AST,CK,CK-MB,LDH,α-HBD,myocardial fiber thickness,and collagen fiber significantly increased,all P<0.01,while following QYN,these indicators improved in the HD group,all P<0.01 or P<0.05.Compared to the CG group,the MG group identified 151 differentially expressed miRNAs,with 42 miRNAs downregulated and 109 miRNAs upregulated;compared to the MG group,the HD group identified 70 differentially expressed miRNAs,40 were downregulated,and 30 were upregulated.The GO functions of differential miRNA target genes are mostly enriched in cardiac development regulation,cardiac contraction control,heart rate regulation,and so on.The most enriched KEGG pathways include the mitogen-activated protein kinase,ErbB,Hippo,forkhead box protein O,and Wnt signaling pathways.CONCLUSION:QYN may protect the cardiac structure and function and minimize cardiac damage caused by GD by regulating relevant target genes and signaling pathways through miRNAs which include miR-206-3p,miR-122-5p,and miR-200a-3p.

    Bushen Huoxue decoction(补肾活血汤)improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and α lpha(v)beta(3)integrin expression

    TANG WeiweiLIU KailiFAN XumeiZHU Li...
    1137-1145页
    查看更多>>摘要:OBJECTIVE:To investigate the mechanism of Bushen Huoxue decoction(补肾活血汤,BSHXD)to treat endometriosis-induced infertility.MEDHODS:The main compounds of BSHXD were determined by high performance liquid chromatography-mass spectrometry(HPLC-MS/MS).The effect of BSHXD on Homeobox A10(HOXA10)and alpha(v)beta(3)(αvβ3)integrin expression of Ishikawa cells,mouse model,and endometriosis-associated infertility women was evaluated by using Western blot analysis,immunohistochemistry and Real-Time quantitative polymerase chain reaction(RT-qPCR).The efficacy of BSHXD on embryo attachment were examined by using the BeWo spheroid and mouse embryo attachment assay.HOXA10 concentration in uterine flushing fluid of endometriosis-associated infertility women treated with BSHXD was measured by Enzyme-Linked immunosorbent assay(ELISA).RESULTS:BSHXD improved BeWo spheroid and mice blastocysts attachment to Ishikawa cells and increased embryo implantation rates in mice and pregnancy rates in women with endometriosis-associated infertility.BSHXD enhanced HOXA10 and αvβ3 integrin expression in Ishikawa cell,endometriosis mouse model,and endometriosis-associated infertility women,which potentially improved endometrial receptivity.CONCLUSIONS:BSHXD could improve endometrial receptivity of endometriosis-associated infertility in a dose-dependent manner by regulating HOXA10 and αvβ3 integrin expression.

    Anti-oxidative and immunological role of Cyclocarya paliurus polysaccharide on the liver injury of diabetic rats

    XIA XichaoXUE ShipengSONG GuoyingLI Bin...
    1146-1152页
    查看更多>>摘要:OBJECTIVE:To investigate the effects of Cyclocarya paliurus(C.paliurus)polysaccharide on the liver injury of diabetic rats.METHODS:Rats were divided into 6 groups,including normal group,model group,control group,low-dose group of C.paliurus polysaccharide treatment,middle-dose group of C.paliurus polysaccharide treatment and high-dose group of C.paliurus polysaccharide treatment.Histological analysis of liver was analyzed using hematoxilin and eosin.Levels of plasma biological parameters and anti-oxidative enzymes were determined by spectrophotometry.Nuclear factor kappa-B-p65(NF-κB p65),tumor necrosis factor-α(TNF-α),interleukins 6(IL-6),IL-1β were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:Compared with that of model group,the glucose level of plasma decreased 62.32%(P<0.01),but glycogen and insulin level increased 1.51 times and 1.27 times in the high-dose group of C.paliurus polysaccharide treatment,respectively.Plasma alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)level decreased 47.47%(P<0.05),43.65%(P<0.05)and 50.51%(P<0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level increased 1.16 times(P<0.01),71.28%(P<0.05),1.29 times(P<0.01)and 87.46%(P<0.05)in the high-dose group of C.paliurus polysaccharide treatment,respectively.Enzyme activities of NF-κB,TNF-α,IL-1βand IL-6 were decreased 39.29%(P<0.05),51.11%(P<0.05),37.42%(P<0.05)and 36.50%(P<0.05),respectively.CONCLUSIONS:Administration of C.paliurus polysaccharide may play a protecting role for liver injury of diabetic rats through lowering glucose,ALT,AST,ALP level,increasing glycogen and insulin level,enhancing the anti-oxidative ability and down-regulating the inflammatory factors expression.

    Transcriptome sequencing-based study on the mechanism of action of Jintiange capsules(金天格胶囊)in regulating synovial mesenchymal stem cells exosomal miRNA and articular chondrocytes mRNA for the treatment of osteoarthritis

    CHEN ZhongyingZHANG XueZHANG XiaofeiZOU Junbo...
    1153-1167页
    查看更多>>摘要:OBJECTIVE:To corroborate the efficacy of Jintiange capsules(JTGs)(金天格胶囊)in the treatment of osteoarthritis(OA)by exploring the potential mechanism of action of synovial mesenchymal stem cell exosomes(SMSC-Exos)and articular chondrocytes(ACs)through transcriptome sequencing(RNA-seq).METHODS:Type Ⅱ collagenase was used to induce OA in rats.The efficacy of JTGs was confirmed by macroscopic observation of articular cartilage,micro-CT observation,and safranin fast green staining.After SMSC-Exos and ACs were qualified,RNA-seq was used to screen differentially expressed miRNAs and mRNAs.The target genes of differentially expressed miRNAs in Synovial mesenchymal stem cells(SMSCs)were predicted based on the multiMiR R package.The co-differentially expressed genes of SMSC-Exos and ACs were obtained by venny 2.1.0.The miRNA-mRNA regulatory network was constructed by Cytoscape software.Based on the OmicShare platform,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed on the mRNA regulated by key miRNAs.Expression trend analysis was performed for co-differentially expressed genes.Correlation analysis was performed on micro-CT efficacy indicators,co-differentially expressed genes mRNA and miRNA.RESULTS:The efficacy of each administration group of JTGs was significant compared with the model group.SMSC-Exos and ACs were identified by their characteristics.The expression of rno-miR-23a-3p,rno-miR-342-3p,rno-miR-146b-5p,rno-miR-501-3p,rno-miR-214-3p was down-regulated in OA pathological state,and the expression of rno-miR-222-3p,rno-miR-30e-3p,rno-miR-676,and rno-miR-192-5p expression was up-regulated,and the expression of all these miRNAs was reversed after the intervention with JTGs containing serum.The co-differentially expressed genes were enriched in the interleukin 17 signaling pathway,tumor necrosis factor signaling pathway,transforming growth factor-β signaling pathway,etc.The expression trends of Ccl7,Akap12,Grem2,Egln3,Arhgdib,Ccl20,Mmp12,Pla2g2a,and Nr4a1 were significant.There was a correlation between micro-CT pharmacodynamic index,mRNA,and miRNA.CONCLUSION:JTGs can improve the degeneration of joint cartilage and achieve the purpose of cartilage protection,which can be used for the treatment of OA.SMSCs-related miRNA expression profiles were significantly altered after the intervention with JTGs containing serum.The 9 co-differentially expressed genes may be the key targets for the efficacy of JTGs in the treatment of OA rats,which can be used for subsequent validation.