首页|Differences in vascular endothelial function and serum proteome between obese people with phlegm-dampness constitution and balanced constitution

Differences in vascular endothelial function and serum proteome between obese people with phlegm-dampness constitution and balanced constitution

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OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese volunteers with the phlegm-dampness constitution or balanced constitution were recruited for this study respectively.The clinical baseline data was collected,and the vascular endothelial function was evaluated using the EndoPATTM.Blood samples were collected for the serum proteome analysis.The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis,correlation analysis,receiver operating characteristic(ROC)curve analysis,and random forest model investigation were conducted.RESULTS:There were no statistical differences found in the baseline data.For vascular endothelial function,the reactive hyperemia index(RHI)of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group(1.46±0.30 vs 2.82±0.78,P＜0.0001),indicating vascular endothelial dysfunction.There are 66 differentially expressed serum proteins between the two groups.apolipoprotein A2(ApoA2),angiotensin-converting enzyme 2(ACE-2),interleukin-33(IL-33),and forkhead box P3(FoxP3)showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.CONCLUSION:Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group.Thus,alterations in the expression levels of key serum proteins,including ApoA2,ACE-2,IL-33,and FoxP3 could serve as potential biomarkers in the obese population at risk of CVD.

obesityphlegm-dampness constitutionvascular endothelial functionproteomereactive hyperemia index

ZHU Linghui、SUN Ziwei、GUAN Yuanyuan、LIU Meiyi、ZHENG Yi、YU Ruoxi、WANG Qi、LI Lingru

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School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China

National Institute of TCM Constitution and Preventive Medicine,Beijing University of Chinese Medicine,Beijing 100029,China

General Program of National Natural Science of China国家重点研发计划National Nonprofit Institute Research Grant for Institute of Basic Theory for Chinese Medicine,CACMS

823743082022YFC2010104YZ-202151

2024

10.19852/j.cnki.jtcm.20231110.005

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(1)
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