首页|Polyphyllin I enhances tumor necrosis factor-related apoptosis-inducing ligand-induced inhibition of human osteosarcoma cell growth via downregulating the Wnt/β-catenin pathway

Polyphyllin I enhances tumor necrosis factor-related apoptosis-inducing ligand-induced inhibition of human osteosarcoma cell growth via downregulating the Wnt/β-catenin pathway

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OBJECTIVE:To investigate the synergistic effects of polyphyllin I(PPI)combined with tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)on the growth of osteosarcoma cells through downregulating the Wnt/β-catenin signaling pathway.METHODS:Cell viability,apoptosis and cell cycle distribution were examined using cell counting kit-8 and flow cytometry assays.The morphology of cancer cells was observed with inverted phase contrast microscope.The migration and invasion abilities were examined by xCELLigence real time cell analysis DP system and transwell assays.The expressions of poly(adenosine diphosphate-ribose)polymerase,C-Myc,Cyclin B1,cyclin-dependent kinases 1,N-cadherin,Vimentin,Active-β-catenin,β-catenin,p-glycogen synthase kinase 3β(GSK-3β)and GSK-3β were determined by Western blotting assay.RESULTS:PPI sensitized TRAIL-induced decrease of viability,migration and invasion,as well as increase of apoptosis and cell cycle arrest of MG-63 and U-2 OS osteosarcoma cells.The synergistic effect of PPI with TRAIL in inhibiting the growth of osteosarcoma cells was at least partially realized through the inactivation of Wnt/β-catenin signaling pathway.CONCLUSION:The combination of PPI and TRAIL is potentially a novel treatment strategy of osteosarcoma.

osteosarcomatumor necrosis factor-related apoptosis-inducing ligandWnt signaling pathwaybeta-cateninpolyphyllin I

CHANG Junli、ZHAO Fulai、SUN Xingyuan、MA Xiaoping、ZHAO Peng、ZHOU Chujie、SHI Binhao、GU Wenchao、WANG Yongjun、YANG Yanping

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Spine Disease Institute,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

Department of Diagnostic and Interventional Radiology,University of Tsukuba,Ibaraki 315-0114,Japan

National Key R&D Program of ChinaNational Nature Science Foundation&&Shanghai Collaborative Innovation Center of Industrial Transformation of Hospital TCM PreparationResearch Projects within Budget of Shanghai University of Traditional Chinese Medicine

2020YFE020160081973877821744082021LK047

2024

10.19852/j.cnki.jtcm.2024.02.002

中医杂志(英文版)
中国中医药学会 中国中医研究院

中医杂志(英文版)

影响因子：0.855
ISSN：0255-2922
年,卷(期)：2024.44(2)
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