首页|Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang(Pogostemonis Herba)based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics
Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang(Pogostemonis Herba)based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
OBJECTIVE:To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.METHODS:High performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope(HPLC-HESI-HRMS)was used to analyze the chemical constituents of the non-volatile ingredients of patchouli.The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide(LPS)induced RAW264.7 cell inflammation model,and the anti-inflammatory mechanism was investigated using multivariate statistical analysis of cell metabolomics.RESULTS:The non-volatile ingredients of patchouli were characterized by HPLC-HESI-HRMS,and 36 flavonoids and 18 other components were identified.These ingredients of patchouli not only had a good protective effect on the LPS-induced inflammation model of RAW264.7 cells,but also regulated the expression levels of arginine,L-leucine,cholesterol,fructose and sorbitol by down-regulating arginine metabolism,aminoacyl-tRNA biosynthesis,polyol/sorbitol pathway,so as to reduce inflammation and reduce cell damage.CONCLUSION:The non-volatile ingredients of patchouli had good anti-inflammatory effect and exerted its curative effect by regulating endogenous metabolic pathway to reduce inflammatory response.
Institute of Control of Chinese Traditional Medicine and Ethnic Medicine,National Institutes for Food and Drug Control,Beijing 102629,China
School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China
Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China
Institute of Chinese Medicine Discipline Construction Project of National Institutes for Food and Drug ControlTraining Fund for Academic Leaders of NIFDCProgram of State Drug Administraion-Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces
万方数据
