Efficacy of Sailuotong(塞络通)on neurovascular unit in amyloid precursor protein/presenilin-1 transgenic mice with Alzheimer's disease

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外文摘要：OBJECTIVE:To discuss the influence of Sailuotong(塞络通,SLT)on the Neurovascular Unit(NVUs)of amyloid precursor protein(APP)/presenilin-1(PS1)mice and evaluate the role of gas supplementation in activating blood circulation during the progression of Alzheimer's disease(AD).METHODS:The mice were allocated into the following nine groups:(a)the C57 Black(C57BL)sham-operated group(control group),(b)ischaemic treatment in C57BL mice(the C57 ischaemic group),(c)the APP/PS1 sham surgery group(APP/PS1 model group),(d)ischaemic treatment in APP/PS1 mice(APP/PS1 ischaemic group),(e)C57BL mice treated with aspirin following ischaemic treatment(C57BL ischaemic+aspirin group),(f)C57BL mice treated with SLT following ischaemic treatment(C57BL ischaemic+SLT group),(g)APP/PS1 mice treated with SLT(APP/PS1+SLT group),(h)APP/PS1 mice treated with donepezil hydrochloride following ischaemic treatment(APP/PS1 ischaemic+donepezil hydrochloride group)and(i)APP/PS1 mice treated with SLT following ischaemic treatment(APP/PS1 ischaemic+SLT group).The ischaemic model was established by operating on the bilateral common carotid arteries and creating a microembolism.The Morris water maze and step-down tests were used to detect the spatial behaviour and memory ability of mice.The hippocampus of each mouse was observed by haematoxylin and eosin(HE)and Congo red staining.The ultrastructure of NVUs in each group was observed by electron microscopy,and various biochemical indicators were detected by enzyme-linked immunosorbent assay(ELSA).The protein expression level was detected by Western blot.The mRNA expression was detected by quantitative real-time polymerase chain reaction(qRT-PCR).RESULTS:The results of the Morris water maze and step-down tests showed that ischemia reduced learning and memory in the mice,which were restored by SLT.The results of HE staining showed that SLT restored the pathological changes of the NVUs.The Congo red staining results revealed that SLT also improved the scattered orange-red sediments in the upper cortex and hippocampus of the APP/PS1 and APP/PS1 ischaemic mice.Furthermore,SLT significantly reduced the content of Aβ,improved the vascular endothelium and repaired the mitochondrial structures.The ELISA detection,western blot detection and qRT-PCR showed that SLT significantly increased the vascular endothelial growth factor(VEGF),angiopoietin and basic fibroblast growth factor,as well as the levels of gene and protein expression of low-density lipoprotein receptor-related protein-1(LRP-1)and VEGF in brain tissue.CONCLUSIONS:By increasing the expression of VEGF,SLT can promote vascular proliferation,up-regulate the expression of LRP-1,promote the clearance of Aβ and improve the cognitive impairment of APP/PS1 mice.These results confirm that SLT can improve AD by promoting vascular proliferation and Aβ clearance to protect the function of NVUs.

外文关键词：

Alzheimer diseaseamyloid beta-protein precursorpresenilin-1mice,transgenicreplenishing Qi and activating bloodneurovascular unitSailuotong

作者：

SUN Linjuan、LI Chengfu、LIU Jiangang、LI Nannan、HAN Fuhua、QIAO Dandan、TAO Zhuang、ZHAN Min、CHEN Wenjie、ZHANG Xiaohui、TONG Chenguang、CHEN Dong、Qi Jiangxia、LIU Yang、LIANG Xiao、ZHENG Xiaoying、ZHANG Yunling

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作者单位：

Department of Neurology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

China Population and Development Research Center,Beijing 100081,China

Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China

Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700,China

Department of Institute of Population Research,Peking University,Beijing 100087,China

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基金：

National Natural Science Foundation of China

项目编号：

81503450

出版年：

2024

DOI：

10.19852/j.cnki.jtcm.20240203.007

中医杂志(英文版)

中国中医药学会中国中医研究院

中医杂志(英文版)

影响因子：0.855

ISSN：0255-2922

年,卷(期)：2024.44(2)

参考文献量36